Gene description for Vim
Gene name vimentin
Gene symbol Vim
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Vim
ExoCarta ExoCarta_81818
Vesiclepedia VP_81818
Entrez Gene 81818
UniProt P31000  
 Vim identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Vim
Molecular Function
    RNA binding GO:0003723 ISO
    double-stranded RNA binding GO:0003725 IEA
    double-stranded RNA binding GO:0003725 ISO
    structural molecule activity GO:0005198 TAS
    structural constituent of cytoskeleton GO:0005200 IBA
    structural constituent of cytoskeleton GO:0005200 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    structural constituent of cytoskeleton GO:0005200 ISS
    structural constituent of eye lens GO:0005212 IEA
    structural constituent of eye lens GO:0005212 ISO
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IPI
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein phosphatase 2A binding GO:0051721 IDA
    molecular adaptor activity GO:0060090 ISO
    scaffold protein binding GO:0097110 IEA
    scaffold protein binding GO:0097110 ISO
    keratin filament binding GO:1990254 IEA
    keratin filament binding GO:1990254 ISO
    protein tyrosine kinase binding GO:1990782 IPI
Biological Process
    response to zinc ion GO:0010043 IEP
    response to selenium ion GO:0010269 IEP
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of epithelial cell migration GO:0010634 ISO
    positive regulation of epithelial cell migration GO:0010634 ISS
    negative regulation of neuron projection development GO:0010977 IEA
    negative regulation of neuron projection development GO:0010977 ISO
    astrocyte development GO:0014002 IEA
    astrocyte development GO:0014002 ISO
    neuron projection development GO:0031175 IEA
    neuron projection development GO:0031175 ISO
    response to estradiol GO:0032355 IEP
    positive regulation of collagen biosynthetic process GO:0032967 IEA
    positive regulation of collagen biosynthetic process GO:0032967 ISO
    response to vitamin D GO:0033280 IEP
    skeletal muscle tissue regeneration GO:0043403 IEP
    regulation of mRNA stability GO:0043488 IEA
    regulation of mRNA stability GO:0043488 ISO
    cellular response to fibroblast growth factor stimulus GO:0044344 IEP
    intermediate filament-based process GO:0045103 ISO
    intermediate filament organization GO:0045109 IBA
    intermediate filament organization GO:0045109 IEA
    intermediate filament organization GO:0045109 ISO
    intermediate filament organization GO:0045109 ISO
    intermediate filament organization GO:0045109 ISS
    fat cell differentiation GO:0045444 IEP
    decidualization GO:0046697 IEP
    regulation of axonogenesis GO:0050770 IDA
    Bergmann glial cell differentiation GO:0060020 IEA
    Bergmann glial cell differentiation GO:0060020 ISO
    positive regulation of glial cell proliferation GO:0060252 IMP
    lens fiber cell development GO:0070307 IEA
    lens fiber cell development GO:0070307 ISO
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to lipopolysaccharide GO:0071222 ISS
    cellular response to muramyl dipeptide GO:0071225 IEA
    cellular response to muramyl dipeptide GO:0071225 ISO
    cellular response to muramyl dipeptide GO:0071225 ISS
    cellular response to type II interferon GO:0071346 IEA
    cellular response to type II interferon GO:0071346 ISO
    regulation of Schwann cell migration GO:1900147 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    peroxisome GO:0005777 IEA
    peroxisome GO:0005777 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 ISO
    intermediate filament GO:0005882 IBA
    intermediate filament GO:0005882 IEA
    intermediate filament GO:0005882 ISO
    intermediate filament GO:0005882 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    nuclear matrix GO:0016363 IEA
    axon GO:0030424 IBA
    axon GO:0030424 IDA
    cell leading edge GO:0031252 IEA
    cell leading edge GO:0031252 ISO
    cell projection GO:0042995 IDA
    cell projection GO:0042995 ISO
    neuron projection GO:0043005 IEA
    neuron projection GO:0043005 ISO
    cell body GO:0044297 IDA
    type III intermediate filament GO:0045098 TAS
    intermediate filament cytoskeleton GO:0045111 ISO
    phagocytic vesicle GO:0045335 IEA
    phagocytic vesicle GO:0045335 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified Vim in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Vim
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gja1  
Affinity Capture-MS Rattus norvegicus
2 PPL 5493
Co-fractionation Homo sapiens
3 Rad23b  
Affinity Capture-MS Rattus norvegicus
4 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
5 DYNLL1 8655
Affinity Capture-MS Homo sapiens
6 Pla2g4a  
Far Western Mus musculus
7 Sumo3  
Affinity Capture-MS Rattus norvegicus
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