Gene description for Eif4b
Gene name eukaryotic translation initiation factor 4B
Gene symbol Eif4b
Other names/aliases 2310046H11Rik
AL024095
C85189
Eif4a2
Species Mus musculus
 Database cross references - Eif4b
ExoCarta ExoCarta_75705
Vesiclepedia VP_75705
Entrez Gene 75705
UniProt Q8BGD9  
 Eif4b identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Eif4b
Molecular Function
    RNA binding GO:0003723 IEA
    translation initiation factor activity GO:0003743 IEA
    RNA strand annealing activity GO:0033592 IBA
    RNA strand-exchange activity GO:0034057 IBA
    ribosomal small subunit binding GO:0043024 IBA
Biological Process
    formation of translation preinitiation complex GO:0001731 IBA
    translational initiation GO:0006413 IEA
    eukaryotic translation initiation factor 4F complex assembly GO:0097010 IBA
Subcellular Localization
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Eif4b in sEVs
1
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Eif4b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Zbtb7b  
Affinity Capture-MS Mus musculus
3 CPSF7 79869
Two-hybrid Homo sapiens
4 Tmem173  
Proximity Label-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Atg16l1  
Affinity Capture-MS Mus musculus
7 Atg12  
Positive Genetic Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
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