Gene description for Sptan1
Gene name spectrin, alpha, non-erythrocytic 1
Gene symbol Sptan1
Other names/aliases A2a
IPF
Spna2
Species Rattus norvegicus
 Database cross references - Sptan1
ExoCarta ExoCarta_64159
Vesiclepedia VP_64159
Entrez Gene 64159
UniProt P16086  
 Sptan1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Sptan1
Molecular Function
    actin binding GO:0003779 IEA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    spectrin binding GO:0030507 IDA
    spectrin binding GO:0030507 ISO
    protein-containing complex binding GO:0044877 IPI
    actin filament binding GO:0051015 IBA
Biological Process
    actin cytoskeleton organization GO:0030036 IBA
    actin filament capping GO:0051693 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    fascia adherens GO:0005916 ISO
    spectrin GO:0008091 TAS
    microtubule cytoskeleton GO:0015630 IEA
    microtubule cytoskeleton GO:0015630 ISO
    membrane GO:0016020 ISO
    lateral plasma membrane GO:0016328 ISO
    Z disc GO:0030018 ISO
    cell junction GO:0030054 IBA
    cortical cytoskeleton GO:0030863 ISO
    cortical actin cytoskeleton GO:0030864 IBA
    cortical actin cytoskeleton GO:0030864 IEA
    cuticular plate GO:0032437 ISO
    protein-containing complex GO:0032991 IDA
    paranodal junction GO:0033010 ISO
    paranode region of axon GO:0033270 ISO
    cell projection GO:0042995 IBA
    intracellular membrane-bounded organelle GO:0043231 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    postsynapse GO:0098794 IDA
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Sptan1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Sptan1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Epb41l2  
Affinity Capture-Western Rattus norvegicus
2 Epb4.1  
Affinity Capture-Western Rattus norvegicus
3 Actn1 81634
Affinity Capture-Western Rattus norvegicus
4 Shank3  
Affinity Capture-Western Rattus norvegicus
5 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
6 Grin2b  
Co-fractionation Rattus norvegicus
View the network image/svg+xml



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