Gene description for Krt1
Gene name keratin 1, type II
Gene symbol Krt1
Other names/aliases Kb1
Species Rattus norvegicus
 Database cross references - Krt1
ExoCarta ExoCarta_300250
Vesiclepedia VP_300250
Entrez Gene 300250
UniProt Q6IMF3  
 Krt1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Krt1
Molecular Function
    carbohydrate binding GO:0030246 IEA
    carbohydrate binding GO:0030246 ISO
    structural constituent of skin epidermis GO:0030280 IBA
    structural constituent of skin epidermis GO:0030280 IEA
    structural constituent of skin epidermis GO:0030280 ISO
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
    protein heterodimerization activity GO:0046982 ISS
Biological Process
    complement activation, lectin pathway GO:0001867 IEA
    complement activation, lectin pathway GO:0001867 ISO
    biological_process GO:0008150 ND
    peptide cross-linking GO:0018149 ISO
    keratinization GO:0031424 IBA
    intermediate filament organization GO:0045109 IBA
    negative regulation of inflammatory response GO:0050728 IEA
    negative regulation of inflammatory response GO:0050728 ISO
    protein heterotetramerization GO:0051290 IEA
    protein heterotetramerization GO:0051290 ISO
    protein heterotetramerization GO:0051290 ISS
    establishment of skin barrier GO:0061436 IEA
    establishment of skin barrier GO:0061436 ISO
Subcellular Localization
    cornified envelope GO:0001533 IEA
    cornified envelope GO:0001533 ISO
    cornified envelope GO:0001533 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 ISO
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 IEA
    keratin filament GO:0045095 ISO
 Experiment description of studies that identified Krt1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Krt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Katna1  
Affinity Capture-MS Rattus norvegicus
2 Sumo3  
Affinity Capture-MS Rattus norvegicus
3 Ap2b1 140670
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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