Gene description for Hspa1l
Gene name heat shock protein 1-like
Gene symbol Hspa1l
Other names/aliases Hsp70-3
Species Rattus norvegicus
 Database cross references - Hspa1l
ExoCarta ExoCarta_24963
Vesiclepedia VP_24963
Entrez Gene 24963
UniProt P55063  
 Hspa1l identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Hspa1l
Molecular Function
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    heat shock protein binding GO:0031072 IBA
    heat shock protein binding GO:0031072 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    protein folding chaperone GO:0044183 IBA
    unfolded protein binding GO:0051082 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    binding of sperm to zona pellucida GO:0007339 ISO
    biological_process GO:0008150 ND
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    positive regulation of protein targeting to mitochondrion GO:1903955 ISO
Subcellular Localization
    zona pellucida receptor complex GO:0002199 ISO
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    COP9 signalosome GO:0008180 ISO
    cell body GO:0044297 ISO
 Experiment description of studies that identified Hspa1l in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Hspa1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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