Gene description for Gnas
Gene name GNAS complex locus
Gene symbol Gnas
Other names/aliases Gnas1
Gnpas
Nesp55
Species Rattus norvegicus
 Database cross references - Gnas
ExoCarta ExoCarta_24896
Vesiclepedia VP_24896
Entrez Gene 24896
UniProt P63095   Q792G6  
 Gnas identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Urine 20535238    
 Gene ontology annotations for Gnas
Molecular Function
    G-protein alpha-subunit binding GO:0001965 IPI
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    G protein activity GO:0003925 IEA
    G protein activity GO:0003925 ISO
    insulin-like growth factor receptor binding GO:0005159 IBA
    insulin-like growth factor receptor binding GO:0005159 IPI
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 TAS
    adenylate cyclase regulator activity GO:0010854 ISO
    adenylate cyclase activator activity GO:0010856 IDA
    adenylate cyclase activator activity GO:0010856 IEA
    adenylate cyclase activator activity GO:0010856 ISO
    adenylate cyclase activator activity GO:0010856 ISS
    protein domain specific binding GO:0019904 IPI
    G-protein beta-subunit binding GO:0031681 IDA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    beta-2 adrenergic receptor binding GO:0031698 IBA
    beta-2 adrenergic receptor binding GO:0031698 IPI
    D1 dopamine receptor binding GO:0031748 IBA
    D1 dopamine receptor binding GO:0031748 IPI
    D1 dopamine receptor binding GO:0031748 ISO
    mu-type opioid receptor binding GO:0031852 IBA
    mu-type opioid receptor binding GO:0031852 IDA
    mu-type opioid receptor binding GO:0031852 IPI
    ionotropic glutamate receptor binding GO:0035255 IBA
    ionotropic glutamate receptor binding GO:0035255 IPI
    alpha-tubulin binding GO:0043014 IDA
    metal ion binding GO:0046872 IEA
    alkylglycerophosphoethanolamine phosphodiesterase activity GO:0047391 IDA
    corticotropin-releasing hormone receptor 1 binding GO:0051430 IBA
    corticotropin-releasing hormone receptor 1 binding GO:0051430 IPI
Biological Process
    skeletal system development GO:0001501 ISO
    tissue homeostasis GO:0001894 IEA
    tissue homeostasis GO:0001894 ISO
    positive regulation of protein phosphorylation GO:0001934 IMP
    endochondral ossification GO:0001958 IEA
    endochondral ossification GO:0001958 ISO
    energy reserve metabolic process GO:0006112 IEA
    energy reserve metabolic process GO:0006112 ISO
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    G protein-coupled receptor signaling pathway GO:0007186 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IDA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISO
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISO
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 IBA
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 IDA
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 IEA
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 ISO
    adenylate cyclase-activating serotonin receptor signaling pathway GO:0007192 ISO
    sensory perception of chemical stimulus GO:0007606 IBA
    positive regulation of cell population proliferation GO:0008284 IDA
    response to xenobiotic stimulus GO:0009410 IEA
    response to xenobiotic stimulus GO:0009410 ISO
    post-embryonic development GO:0009791 ISO
    regulation of signal transduction GO:0009966 ISO
    positive regulation of sodium ion transport GO:0010765 IMP
    positive regulation of insulin secretion GO:0032024 IEA
    positive regulation of insulin secretion GO:0032024 ISO
    response to prostaglandin E GO:0034695 ISO
    embryonic hindlimb morphogenesis GO:0035116 IEA
    embryonic hindlimb morphogenesis GO:0035116 ISO
    multicellular organism growth GO:0035264 IEA
    multicellular organism growth GO:0035264 ISO
    negative regulation of multicellular organism growth GO:0040015 ISO
    post-embryonic body morphogenesis GO:0040032 IEA
    post-embryonic body morphogenesis GO:0040032 ISO
    skin development GO:0043588 IEA
    skin development GO:0043588 ISO
    positive regulation of osteoblast differentiation GO:0045669 IEA
    positive regulation of osteoblast differentiation GO:0045669 ISO
    positive regulation of osteoclast differentiation GO:0045672 IEA
    positive regulation of osteoclast differentiation GO:0045672 ISO
    negative regulation of blood pressure GO:0045776 IMP
    developmental growth GO:0048589 ISO
    embryonic cranial skeleton morphogenesis GO:0048701 IEA
    embryonic cranial skeleton morphogenesis GO:0048701 ISO
    cognition GO:0050890 ISO
    cartilage development GO:0051216 IEA
    cartilage development GO:0051216 ISO
    bone development GO:0060348 ISO
    hair follicle placode formation GO:0060789 ISO
    platelet aggregation GO:0070527 ISO
    response to parathyroid hormone GO:0071107 IEA
    response to parathyroid hormone GO:0071107 ISO
    cellular response to glucagon stimulus GO:0071377 IEA
    cellular response to glucagon stimulus GO:0071377 ISO
    cellular response to prostaglandin E stimulus GO:0071380 IDA
    genomic imprinting GO:0071514 IEA
    genomic imprinting GO:0071514 ISO
    cellular response to catecholamine stimulus GO:0071870 IDA
    adenylate cyclase-activating adrenergic receptor signaling pathway GO:0071880 ISO
    adenylate cyclase-activating adrenergic receptor signaling pathway GO:0071880 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 IEA
    positive regulation of cold-induced thermogenesis GO:0120162 ISO
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    regulation of parathyroid hormone secretion GO:2000828 IEA
    regulation of parathyroid hormone secretion GO:2000828 ISO
Subcellular Localization
    ruffle GO:0001726 IDA
    extracellular region GO:0005576 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 IDA
    heterotrimeric G-protein complex GO:0005834 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    synaptic vesicle GO:0008021 IEA
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    apical plasma membrane GO:0016324 IEA
    COPI-coated Golgi to ER transport vesicle GO:0030142 IDA
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    trans-Golgi network membrane GO:0032588 ISO
    sarcolemma GO:0042383 IDA
    neuronal cell body GO:0043025 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    recycling endosome GO:0055037 IDA
 Experiment description of studies that identified Gnas in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gnas
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo21  
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Gnas is involved
No pathways found





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