Gene description for AI314180
Gene name expressed sequence AI314180
Gene symbol AI314180
Other names/aliases AW558785
BB181316
mKIAA0368
Species Mus musculus
 Database cross references - AI314180
ExoCarta ExoCarta_230249
Vesiclepedia VP_230249
Entrez Gene 230249
UniProt Q6PDI5  
 AI314180 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for AI314180
Molecular Function
    molecular adaptor activity GO:0060090 IBA
    molecular adaptor activity GO:0060090 IEA
    proteasome binding GO:0070628 IEA
    proteasome binding GO:0070628 ISO
Biological Process
    ERAD pathway GO:0036503 IBA
    ERAD pathway GO:0036503 IEA
    ERAD pathway GO:0036503 ISO
    proteasome assembly GO:0043248 IEA
Subcellular Localization
    proteasome complex GO:0000502 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    late endosome GO:0005770 ISO
    multivesicular body GO:0005771 ISO
    multivesicular body GO:0005771 ISS
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    COPII-coated ER to Golgi transport vesicle GO:0030134 IEA
    COPII-coated ER to Golgi transport vesicle GO:0030134 ISO
    endocytic vesicle GO:0030139 ISO
    endocytic vesicle GO:0030139 ISS
    cytoplasmic vesicle GO:0031410 ISO
 Experiment description of studies that identified AI314180 in sEVs
1
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AI314180
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Affinity Capture-MS Homo sapiens
2 Ubr4 69116
Co-fractionation Mus musculus
3 Lars 107045
Co-fractionation Mus musculus
4 3110002H16Rik  
Co-fractionation Mus musculus
5 Vps36 70160
Co-fractionation Mus musculus
6 Dhx29  
Co-fractionation Mus musculus
7 Psma2 19166
Co-fractionation Mus musculus
8 Rps4x 20102
Co-fractionation Mus musculus
9 Rps2 16898
Co-fractionation Mus musculus
10 COBLL1 22837
Affinity Capture-MS Homo sapiens
11 PHF3  
Affinity Capture-MS Homo sapiens
12 Myo1b  
Co-fractionation Mus musculus
13 Rpl6 19988
Co-fractionation Mus musculus
14 Txnl1 53382
Co-fractionation Mus musculus
15 Gareml  
Co-fractionation Mus musculus
16 Rpl35a 57808
Co-fractionation Mus musculus
17 Lrrc59  
Co-fractionation Mus musculus
18 Dnajb9  
Co-fractionation Mus musculus
19 March5  
Co-fractionation Mus musculus
20 PHACTR4 65979
Affinity Capture-MS Homo sapiens
21 Psmc4 23996
Co-fractionation Mus musculus
22 Atp9a  
Co-fractionation Mus musculus
23 Rps3a1 20091
Co-fractionation Mus musculus
24 Rps14 20044
Co-fractionation Mus musculus
25 Ptchd2  
Co-fractionation Mus musculus
26 Psma6 26443
Co-fractionation Mus musculus
27 ANXA2 302
Affinity Capture-MS Homo sapiens
28 Rpl15 66480
Co-fractionation Mus musculus
29 Top1 21969
Co-fractionation Mus musculus
30 Dock11  
Co-fractionation Mus musculus
31 Fancd2  
Affinity Capture-MS Mus musculus
32 Lipt2  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which AI314180 is involved
No pathways found





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