Gene description for Ptgir
Gene name prostaglandin I receptor (IP)
Gene symbol Ptgir
Other names/aliases IP
PGI2
Species Mus musculus
 Database cross references - Ptgir
ExoCarta ExoCarta_19222
Vesiclepedia VP_19222
Entrez Gene 19222
UniProt P43252  
 Ptgir identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Ptgir
Molecular Function
    G protein-coupled receptor activity GO:0004930 IEA
    prostacyclin receptor activity GO:0016501 IBA
Biological Process
    inflammatory response GO:0006954 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISO
    positive regulation of cytosolic calcium ion concentration GO:0007204 IBA
    negative regulation of platelet-derived growth factor receptor signaling pathway GO:0010642 IMP
    response to lipopolysaccharide GO:0032496 IMP
    negative regulation of smooth muscle cell proliferation GO:0048662 IBA
    negative regulation of smooth muscle cell proliferation GO:0048662 IMP
    negative regulation of smooth muscle cell proliferation GO:0048662 ISO
Subcellular Localization
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Ptgir in sEVs
1
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ptgir
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gna11 14672
Affinity Capture-Western Mus musculus
2 Gnai1  
Affinity Capture-Western Mus musculus
3 Gnas 14683
Affinity Capture-Western Mus musculus
4 PDZK1 5174
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
View the network image/svg+xml



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