Experiment description of studies that identified Pdia4 in sEVs

1 Experiment ID 35 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|CD81|CD63 Enriched markers ✔ HSPA5 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19367702     Organism Rattus norvegicus Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM" Journal name JPR Publication year 2008 Sample Hepatocytes Sample name Hepatocytes Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting

2 Experiment ID 97 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

Protein-protein interactions for Pdia4

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Hsp90b1 362862 Affinity Capture-Western Rattus norvegicus 2 Hyou1 192235 Affinity Capture-Western Rattus norvegicus 3 Hspa5 25617 Affinity Capture-Western Rattus norvegicus Affinity Capture-Western Rattus norvegicus 4 Thbs1 445442 Affinity Capture-Western Rattus norvegicus 5 Tg   Affinity Capture-Western Rattus norvegicus Affinity Capture-Western Rattus norvegicus View the network

Pathways in which Pdia4 is involved

No pathways found