Gene description for Gne
Gene name glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Gene symbol Gne
Other names/aliases Uae1
Species Rattus norvegicus
 Database cross references - Gne
ExoCarta ExoCarta_114711
Vesiclepedia VP_114711
Entrez Gene 114711
UniProt O35826  
 Gne identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Gne
Molecular Function
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 IBA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 IDA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 IEA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 ISO
    N-acylmannosamine kinase activity GO:0009384 IBA
    N-acylmannosamine kinase activity GO:0009384 IDA
    N-acylmannosamine kinase activity GO:0009384 IEA
    N-acylmannosamine kinase activity GO:0009384 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    N-acetylglucosamine biosynthetic process GO:0006045 IEA
    UDP-N-acetylglucosamine metabolic process GO:0006047 IEA
    CMP-N-acetylneuraminate biosynthetic process GO:0006055 IEA
    CMP-N-acetylneuraminate biosynthetic process GO:0006055 ISO
    N-acetylneuraminate biosynthetic process GO:0046380 IC
    N-acetylneuraminate biosynthetic process GO:0046380 IEA
    N-acetylneuraminate biosynthetic process GO:0046380 ISO
    N-acetylneuraminate biosynthetic process GO:0046380 TAS
    carbohydrate phosphorylation GO:0046835 IEA
    glycosylation GO:0070085 IEA
    glycosylation GO:0070085 ISO
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Gne in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gne
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZBTB16  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 KIAA1549  
Two-hybrid Homo sapiens
3 CRMP1 1400
Two-hybrid Homo sapiens
4 Crmp1  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml



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