Gene description for FLOT1
Gene name flotillin 1
Gene symbol FLOT1
Other names/aliases -
Species Homo sapiens
 Database cross references - FLOT1
ExoCarta ExoCarta_10211
Vesiclepedia VP_10211
Entrez Gene 10211
HGNC 3757
MIM 606998
UniProt O75955  
 FLOT1 identified in sEVs derived from the following tissue/cell type
Aqueous humor 21362503    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34112803    
Breast cancer cells 34112803    
Breast cancer cells 35939247    
Breast cancer cells 35939247    
Breast cancer cells 35939247    
Breast cancer cells 35939247    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 34887515    
Breast cancer cells 34887515    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colon cancer cells 34887515    
Colon cancer cells 34887515    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Colorectal cancer cells 34887515    
Colorectal cancer cells 34887515    
Colorectal cancer cells 36068649    
Colorectal cancer cells 36068649    
Colorectal cancer cells 36303008    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Endothelial cells 26027894    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lung cancer cells 34887515    
Lung cancer cells 33686255    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 26176991    
Melanoma cells 34789738    
Melanoma cells 34789738    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal colon fibroblasts 36068649    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34887515    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Plasma 34112803    
Plasma 34887515    
Plasma 34887515    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Prostate cancer cells 25519911    
Prostate cancer cells 25519911    
Prostate cancer cells 25519911    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Retinal endothelial cells 34887515    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Tabecular meshwork cells 21362503    
Thymus 23844026    
Urine 15326289    
Urine 19056867    
Urine 21362503    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for FLOT1
Molecular Function
    protease binding GO:0002020 IBA
    protease binding GO:0002020 IPI
    protein binding GO:0005515 IPI
    ionotropic glutamate receptor binding GO:0035255 IEA
Biological Process
    positive regulation of cytokine production GO:0001819 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    regulation of receptor internalization GO:0002090 IBA
    regulation of receptor internalization GO:0002090 IMP
    axonogenesis GO:0007409 IEA
    extracellular matrix disassembly GO:0022617 IMP
    positive regulation of protein binding GO:0032092 IMP
    positive regulation of synaptic transmission, dopaminergic GO:0032226 ISS
    positive regulation of interferon-beta production GO:0032728 IMP
    dsRNA transport GO:0033227 IMP
    positive regulation of heterotypic cell-cell adhesion GO:0034116 IMP
    positive regulation of toll-like receptor 3 signaling pathway GO:0034141 IMP
    regulation of toll-like receptor 4 signaling pathway GO:0034143 IMP
    response to endoplasmic reticulum stress GO:0034976 IMP
    regulation of Rho protein signal transduction GO:0035023 IEA
    intracellular signal transduction GO:0035556 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    plasma membrane raft assembly GO:0044854 IMP
    plasma membrane raft organization GO:0044857 IMP
    positive regulation of endocytosis GO:0045807 IBA
    positive regulation of endocytosis GO:0045807 IMP
    positive regulation of skeletal muscle tissue development GO:0048643 IMP
    protein stabilization GO:0050821 IMP
    regulation of neurotransmitter uptake GO:0051580 IDA
    regulation of neurotransmitter uptake GO:0051580 IMP
    protein kinase C signaling GO:0070528 IBA
    cellular response to exogenous dsRNA GO:0071360 IMP
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IMP
    positive regulation of myoblast fusion GO:1901741 IMP
    positive regulation of cell junction assembly GO:1901890 IBA
    positive regulation of cell junction assembly GO:1901890 IMP
    positive regulation of cell-cell adhesion mediated by cadherin GO:2000049 IBA
    positive regulation of cell-cell adhesion mediated by cadherin GO:2000049 IMP
Subcellular Localization
    uropod GO:0001931 IDA
    lysosomal membrane GO:0005765 HDA
    endosome GO:0005768 IDA
    early endosome GO:0005769 IDA
    microtubule organizing center GO:0005815 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    caveola GO:0005901 TAS
    cell-cell junction GO:0005911 IDA
    adherens junction GO:0005912 IDA
    focal adhesion GO:0005925 HDA
    COP9 signalosome GO:0008180 IDA
    external side of plasma membrane GO:0009897 IEA
    membrane GO:0016020 HDA
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IDA
    flotillin complex GO:0016600 IBA
    flotillin complex GO:0016600 IDA
    lamellipodium GO:0030027 IDA
    cortical actin cytoskeleton GO:0030864 IDA
    cytoplasmic vesicle GO:0031410 IBA
    cytoplasmic vesicle GO:0031410 IDA
    centriolar satellite GO:0034451 IDA
    sarcolemma GO:0042383 IEA
    melanosome GO:0042470 IEA
    cell-cell contact zone GO:0044291 IDA
    membrane raft GO:0045121 IDA
    presynaptic active zone GO:0048786 IEA
    extracellular exosome GO:0070062 HDA
    dopaminergic synapse GO:0098691 IDA
    dopaminergic synapse GO:0098691 IMP
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified FLOT1 in sEVs
1
Experiment ID 120
MISEV standards
✘ Biophysical techniques
✔
GAPDH|CD9|CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21362503    
Organism Homo sapiens
Experiment description Protein profile of exosomes from trabecular meshwork cells.
Authors "Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL."
Journal name JOP
Publication year 2011
Sample Aqueous humor
Sample name Aqueous humor - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
6
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
11
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 429
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
17
Experiment ID 430
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 431
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 4
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
19
Experiment ID 432
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 433
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 522
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|CD9
Enriched markers
✔
GOLGA2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Homo sapiens
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 523
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|CD9
Enriched markers
✔
GOLGA2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Homo sapiens
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
23
Experiment ID 679
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 35939247    
Organism Homo sapiens
Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors "Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name Clin Exp Metastasis
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-231T
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
24
Experiment ID 680
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 35939247    
Organism Homo sapiens
Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors "Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name Clin Exp Metastasis
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-231T
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 681
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 35939247    
Organism Homo sapiens
Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors "Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name Clin Exp Metastasis
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-435
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 682
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 35939247    
Organism Homo sapiens
Experiment description Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors "Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name Clin Exp Metastasis
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-435
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 924
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|SDCBP|CD81|RAB35|LAMP2|FLOT1|TSG101|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 926
MISEV standards
✘ Biophysical techniques
✔
CD9|FLOT1|TSG101|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 930
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|FLOT1|TSG101|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
30
Experiment ID 932
MISEV standards
✘ Biophysical techniques
✔
CD9|FLOT1|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
31
Experiment ID 933
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|FLOT1|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
32
Experiment ID 1181
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 1182
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
34
Experiment ID 1184
MISEV standards
✘ Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 5
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
35
Experiment ID 1185
MISEV standards
✘ Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
36
Experiment ID 1186
MISEV standards
✘ Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 7
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
37
Experiment ID 1187
MISEV standards
✘ Biophysical techniques
✔
FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 8
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
38
Experiment ID 1188
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 9
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
39
Experiment ID 1189
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 1
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density 1.05 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
40
Experiment ID 1190
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 3
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
41
Experiment ID 1191
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 4
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
42
Experiment ID 1192
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 5
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
43
Experiment ID 1193
MISEV standards
✘ Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
44
Experiment ID 1194
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 7
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
45
Experiment ID 1195
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 8
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
46
Experiment ID 1196
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 9
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
47
Experiment ID 1197
MISEV standards
✘ Biophysical techniques
✔
TSG101|CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
48
Experiment ID 1198
MISEV standards
✘ Biophysical techniques
✔
TSG101|CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
49
Experiment ID 1206
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
ELISA
50
Experiment ID 1210
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name LM2-4175
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
51
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
52
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
53
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
54
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
55
Experiment ID 1213
MISEV standards
✘ Biophysical techniques
✔
FLOT1|CD9|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colon cancer cells
Sample name DiFi-derived EGFR/CD81-positive sEVs - dim (FAVS)
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
56
Experiment ID 1214
MISEV standards
✘ Biophysical techniques
✔
EPCAM|FLOT1|CD9|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colon cancer cells
Sample name DiFi-derived EGFR/CD81-positive sEVs - bright (FAVS)
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
57
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
58
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
59
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
60
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
61
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
62
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
63
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
64
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
65
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
66
Experiment ID 1202
MISEV standards
✘ Biophysical techniques
✔
SDCBP|CD9|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
67
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
68
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
69
Experiment ID 1207
MISEV standards
✘ Biophysical techniques
✔
SDCBP|CD9|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name HCA-7 SC
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
70
Experiment ID 1340
MISEV standards
Biophysical techniques
✔
CD9|TSG101|CD63|FLOT1|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36068649    
Organism Homo sapiens
Experiment description Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway
Authors "Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G"
Journal name J Extracell Vesicles
Publication year 2022
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
71
Experiment ID 1341
MISEV standards
Biophysical techniques
✔
CD9|TSG101|CD63|FLOT1|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36068649    
Organism Homo sapiens
Experiment description Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway
Authors "Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G"
Journal name J Extracell Vesicles
Publication year 2022
Sample Colorectal cancer cells
Sample name HCT116
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
72
Experiment ID 1361
MISEV standards
Biophysical techniques
✔
TSG101|CD63|FLOT1|LAMP1|CD24|EPCAM|Alix
Enriched markers
✔
HSP90AA1
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Colorectal cancer cells
Sample name HCT116
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
MORPH
ELISA
73
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
74
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
75
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
76
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
77
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
78
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
79
Experiment ID 421
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
80
Experiment ID 422
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
81
Experiment ID 423
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
82
Experiment ID 424
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 4
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
83
Experiment ID 425
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
84
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
85
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
86
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
87
Experiment ID 1348
MISEV standards
Biophysical techniques
✔
FLOT1|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
88
Experiment ID 1349
MISEV standards
Biophysical techniques
✔
FLOT1|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
89
Experiment ID 1350
MISEV standards
Biophysical techniques
✔
FLOT1|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
90
Experiment ID 1351
MISEV standards
Biophysical techniques
✔
FLOT1|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
91
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
92
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
93
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
94
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
95
Experiment ID 1201
MISEV standards
✘ Biophysical techniques
✔
SDCBP|CD9|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Lung cancer cells
Sample name Calu-3
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
96
Experiment ID 1324
MISEV standards
Biophysical techniques
✔
GAPDH|CD63|LAMP1|FLOT1|TSG101|Alix
Enriched markers
✔
GAPDH|HSP90AA1
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33686255    
Organism Homo sapiens
Experiment description Extracellular vesicle drug occupancy enables real-time monitoring of targeted cancer therapy.
Authors "Pan S, Zhang Y, Natalia A, Lim CZJ, Ho NRY, Chowbay B, Loh TP, Tam JKC, Shao H."
Journal name Nat Nanotechnol
Publication year 2021
Sample Lung cancer cells
Sample name H3255
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
97
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
98
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
99
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
100
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
101
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
102
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
103
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
104
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
105
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
106
Experiment ID 265
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26176991    
Organism Homo sapiens
Experiment description Small RNA deep sequencing discriminates subsets of extracellular vesicles released by melanoma cells - evidence of unique microRNA cargos
Authors "Lunavat TR, Cheng L, Kim DK, Bhadury J, Jang SC, Lasser C, Sharples RA, Lopez MD, Nilsson J, Gho YS, Hill AF, Lotvall J."
Journal name RNA Biology
Publication year 2015
Sample Melanoma cells
Sample name MML-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
snRNA
snoRNA
mtRNA
Ro associated Y-RNA
MiscRNA
Methods used in the study Western blotting
RNA deep sequencing
107
Experiment ID 1091
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34789738    
Organism Homo sapiens
Experiment description P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells
Authors "Pegoraro A, De Marchi E, Ferracin M, Orioli E, Zanoni M, Bassi C, Tesei A, Capece M, Dika E, Negrini M, Di Virgilio F, Adinolfi E."
Journal name Cell Death Dis
Publication year 2021
Sample Melanoma cells
Sample name SK-MEL-28 - 100k pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
RT-PCR
108
Experiment ID 1092
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34789738    
Organism Homo sapiens
Experiment description P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells
Authors "Pegoraro A, De Marchi E, Ferracin M, Orioli E, Zanoni M, Bassi C, Tesei A, Capece M, Dika E, Negrini M, Di Virgilio F, Adinolfi E."
Journal name Cell Death Dis
Publication year 2021
Sample Melanoma cells
Sample name Ma-Mel-19 - 100k pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
RT-PCR
109
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
110
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
111
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
112
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
113
Experiment ID 1339
MISEV standards
Biophysical techniques
✔
CD9|TSG101|CD63|FLOT1|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36068649    
Organism Homo sapiens
Experiment description Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway
Authors "Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G"
Journal name J Extracell Vesicles
Publication year 2022
Sample Normal colon fibroblasts
Sample name CCD18Co
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
114
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
115
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
116
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
117
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
118
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
119
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
120
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
121
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
122
Experiment ID 437
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Fraction 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
123
Experiment ID 438
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Fraction 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
124
Experiment ID 439
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Fraction 4
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
125
Experiment ID 440
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
126
Experiment ID 1205
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
127
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
128
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
129
Experiment ID 524
MISEV standards
✘ Biophysical techniques
✔
FLOT1|CD9
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Homo sapiens
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Plasma
Sample name Pooled plasma from healthy subjects and breast cancer patients
Isolation/purification methods Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
130
Experiment ID 1204
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Plasma
Sample name Colorectal cancer patients
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
ELISA
131
Experiment ID 1209
MISEV standards
✘ Biophysical techniques
✔
FLOT1|SDCBP
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Plasma
Sample name Control individuals
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
132
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
133
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
134
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
135
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
136
Experiment ID 245
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|CD9|CD63|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25519911    
Organism Homo sapiens
Experiment description The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors "Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A."
Journal name J Biol Chem
Publication year 2015
Sample Prostate cancer cells
Sample name PC-3 - Ethanol treated (control)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Lipids
Protein
Methods used in the study Western blotting
Mass spectrometry
137
Experiment ID 246
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|CD9|CD63|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25519911    
Organism Homo sapiens
Experiment description The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors "Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A."
Journal name J Biol Chem
Publication year 2015
Sample Prostate cancer cells
Sample name PC-3 - sn-1-O-hexadecylglycerol treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Lipids
Methods used in the study Western blotting
Mass spectrometry
138
Experiment ID 247
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25519911    
Organism Homo sapiens
Experiment description The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors "Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A."
Journal name J Biol Chem
Publication year 2015
Sample Prostate cancer cells
Sample name PC-3 - DL- palmitin treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Lipids
Methods used in the study Western blotting
Mass spectrometry
139
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
140
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
141
Experiment ID 1208
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Retinal endothelial cells
Sample name HREC
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
142
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
143
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
144
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
145
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
146
Experiment ID 836
MISEV standards
✘ Biophysical techniques
✔
LAMP2|CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
147
Experiment ID 837
MISEV standards
✘ Biophysical techniques
✔
LAMP2|CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
148
Experiment ID 838
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
149
Experiment ID 839
MISEV standards
✘ Biophysical techniques
✔
LAMP2|CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
150
Experiment ID 840
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
151
Experiment ID 841
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
152
Experiment ID 842
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
153
Experiment ID 843
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
154
Experiment ID 844
MISEV standards
✘ Biophysical techniques
✔
LAMP2|CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
155
Experiment ID 845
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
156
Experiment ID 848
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
157
Experiment ID 849
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
158
Experiment ID 850
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
159
Experiment ID 851
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
160
Experiment ID 852
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
161
Experiment ID 853
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
162
Experiment ID 854
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
163
Experiment ID 855
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
164
Experiment ID 856
MISEV standards
✘ Biophysical techniques
✔
FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
165
Experiment ID 857
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
166
Experiment ID 858
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
167
Experiment ID 859
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
168
Experiment ID 860
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
169
Experiment ID 863
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
170
Experiment ID 864
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
171
Experiment ID 865
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
172
Experiment ID 866
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
173
Experiment ID 867
MISEV standards
✘ Biophysical techniques
✔
CD63|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
174
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
175
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
176
Experiment ID 118
MISEV standards
✘ Biophysical techniques
✔
SDCBP|GAPDH|CD9|CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21362503    
Organism Homo sapiens
Experiment description Protein profile of exosomes from trabecular meshwork cells.
Authors "Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL."
Journal name JOP
Publication year 2011
Sample Tabecular meshwork cells
Sample name Tabecular meshwork cells
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.07-1.2 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
177
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
178
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
179
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
180
Experiment ID 119
MISEV standards
✘ Biophysical techniques
✔
SDCBP|CD9|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21362503    
Organism Homo sapiens
Experiment description Protein profile of exosomes from trabecular meshwork cells.
Authors "Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL."
Journal name JOP
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
181
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
182
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
183
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
184
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
185
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FLOT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ISG15 9636
Affinity Capture-MS Homo sapiens
2 WDR6 11180
Proximity Label-MS Homo sapiens
3 UTRN 7402
Proximity Label-MS Homo sapiens
4 RASSF8 11228
Proximity Label-MS Homo sapiens
5 NPLOC4 55666
Co-fractionation Homo sapiens
6 ETV6  
Affinity Capture-MS Homo sapiens
7 STAT5B 6777
Affinity Capture-MS Homo sapiens
8 LDHB 3945
Proximity Label-MS Homo sapiens
9 LLGL1 3996
Proximity Label-MS Homo sapiens
10 PKP2 5318
Proximity Label-MS Homo sapiens
11 JPH1 56704
Proximity Label-MS Homo sapiens
12 ABCA1 19
Affinity Capture-Western Homo sapiens
13 KIF20A 10112
Affinity Capture-MS Homo sapiens
14 C1orf226  
Proximity Label-MS Homo sapiens
15 UBC 7316
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
16 VHL  
Two-hybrid Homo sapiens
17 SEPT2 4735
Proximity Label-MS Homo sapiens
18 ELF4 2000
Affinity Capture-MS Homo sapiens
19 CCDC90B  
Proximity Label-MS Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 SEPT9 10801
Proximity Label-MS Homo sapiens
22 PTRH2 51651
Co-fractionation Homo sapiens
23 KIF23 9493
Affinity Capture-MS Homo sapiens
24 PLCH1  
Proximity Label-MS Homo sapiens
25 UNC5B 219699
Proximity Label-MS Homo sapiens
26 SEL1L 6400
Co-fractionation Homo sapiens
27 CALD1 800
Affinity Capture-MS Homo sapiens
28 SLC9A9 285195
Affinity Capture-MS Homo sapiens
29 NFATC3 4775
Affinity Capture-MS Homo sapiens
30 MARCH5  
Proximity Label-MS Homo sapiens
31 RASAL2 9462
Proximity Label-MS Homo sapiens
32 BOD1L1 259282
Cross-Linking-MS (XL-MS) Homo sapiens
33 Flot2 14252
Affinity Capture-MS Mus musculus
34 SEPT7 989
Proximity Label-MS Homo sapiens
35 LMAN2 10960
Co-fractionation Homo sapiens
36 MECP2 4204
Affinity Capture-MS Homo sapiens
37 Vps28 66914
Affinity Capture-MS Mus musculus
38 SLC12A7 10723
Proximity Label-MS Homo sapiens
39 ZFYVE9  
Proximity Label-MS Homo sapiens
40 ARHGEF4  
Affinity Capture-MS Homo sapiens
41 EXOC3 11336
Proximity Label-MS Homo sapiens
42 SORBS1 10580
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
43 NOTCH2 4853
Proximity Label-MS Homo sapiens
44 EMC1 23065
Co-fractionation Homo sapiens
45 AP3M1 26985
Proximity Label-MS Homo sapiens
46 DNAL4  
Affinity Capture-MS Homo sapiens
47 VAV1 7409
Affinity Capture-MS Homo sapiens
48 SORBS2  
Affinity Capture-Western Homo sapiens
49 ILVBL 10994
Co-fractionation Homo sapiens
50 MCAM 4162
Proximity Label-MS Homo sapiens
51 EFR3B  
Proximity Label-MS Homo sapiens
52 CERS2 29956
Co-fractionation Homo sapiens
53 CDH1 999
Proximity Label-MS Homo sapiens
54 YAP1 10413
Affinity Capture-MS Homo sapiens
55 MYH9 4627
Affinity Capture-MS Homo sapiens
56 SYNJ2BP 55333
Affinity Capture-MS Homo sapiens
57 DTNB 1838
Proximity Label-MS Homo sapiens
58 GORASP2 26003
Affinity Capture-MS Homo sapiens
59 Coro1c 23790
Affinity Capture-MS Mus musculus
60 MPP7 143098
Proximity Label-MS Homo sapiens
61 FLOT2 2319
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
62 PICALM 8301
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
63 MPDZ  
Proximity Label-MS Homo sapiens
64 SRPK1 6732
Biochemical Activity Homo sapiens
65 ANLN 54443
Affinity Capture-MS Homo sapiens
66 NCKAP5L  
Proximity Label-MS Homo sapiens
67 DST 667
Proximity Label-MS Homo sapiens
68 CSNK1G3 1456
Affinity Capture-MS Homo sapiens
69 PALM2  
Proximity Label-MS Homo sapiens
70 EPB41L1 2036
Proximity Label-MS Homo sapiens
71 ARHGAP32  
Proximity Label-MS Homo sapiens
72 FAM83B  
Proximity Label-MS Homo sapiens
73 FAM129B 64855
Proximity Label-MS Homo sapiens
74 Cd2ap 12488
Affinity Capture-MS Mus musculus
75 SDHA 6389
Affinity Capture-MS Homo sapiens
76 SCAMP1 9522
Proximity Label-MS Homo sapiens
77 ERBB2IP 55914
Proximity Label-MS Homo sapiens
78 ANXA1 301
Protein-peptide Homo sapiens
79 DLG1 1739
Proximity Label-MS Homo sapiens
80 GOLT1B 51026
Affinity Capture-MS Homo sapiens
81 CCDC8  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
82 MAP4K4 9448
Proximity Label-MS Homo sapiens
83 LMTK2 22853
Proximity Label-MS Homo sapiens
84 MYO19  
Affinity Capture-MS Homo sapiens
85 TEAD2  
Affinity Capture-MS Homo sapiens
86 FN1 2335
Affinity Capture-MS Homo sapiens
87 RPA3 6119
Proximity Label-MS Homo sapiens
88 DLST 1743
Affinity Capture-MS Homo sapiens
89 ABCC1 4363
Co-fractionation Homo sapiens
90 SORBS3 10174
Reconstituted Complex Homo sapiens
91 DZIP1  
Proximity Label-MS Homo sapiens
92 CRYBG3  
Proximity Label-MS Homo sapiens
93 CTNNA1 1495
Proximity Label-MS Homo sapiens
94 KIDINS220 57498
Proximity Label-MS Homo sapiens
95 USP6NL 9712
Proximity Label-MS Homo sapiens
96 IRF1  
Affinity Capture-MS Homo sapiens
97 PDE8A 5151
Proximity Label-MS Homo sapiens
98 SOX2  
Affinity Capture-MS Homo sapiens
99 STAT4  
Affinity Capture-MS Homo sapiens
100 YBX1 4904
Co-fractionation Homo sapiens
101 GPRIN3  
Proximity Label-MS Homo sapiens
102 PSD3 23362
Proximity Label-MS Homo sapiens
103 SHBG 6462
Two-hybrid Homo sapiens
104 PLEC 5339
Proximity Label-MS Homo sapiens
105 MACF1 23499
Proximity Label-MS Homo sapiens
106 LCK 3932
Proximity Label-MS Homo sapiens
107 S100A10 6281
Co-fractionation Homo sapiens
108 RAB11FIP1 80223
Proximity Label-MS Homo sapiens
109 HM13 81502
Co-fractionation Homo sapiens
110 PHACTR4 65979
Proximity Label-MS Homo sapiens
111 RAB11A 8766
Affinity Capture-MS Homo sapiens
112 PLEKHA7 144100
Proximity Label-MS Homo sapiens
113 EPHA2 1969
Proximity Label-MS Homo sapiens
114 CUL7 9820
Affinity Capture-MS Homo sapiens
115 RAB5C 5878
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
116 MRPL55  
Co-fractionation Homo sapiens
117 VANGL1 81839
Proximity Label-MS Homo sapiens
118 NDUFS6  
Co-fractionation Homo sapiens
119 CTNND1 1500
Proximity Label-MS Homo sapiens
120 SEC61A1 29927
Proximity Label-MS Homo sapiens
121 ARHGEF25 115557
Affinity Capture-MS Homo sapiens
122 Tmed2 56334
Affinity Capture-MS Mus musculus
123 IARS2 55699
Proximity Label-MS Homo sapiens
124 Spast  
Affinity Capture-MS Mus musculus
125 FBXO7 25793
Affinity Capture-MS Homo sapiens
126 ARHGAP39  
Proximity Label-MS Homo sapiens
127 NOS1AP  
Proximity Label-MS Homo sapiens
128 NUP188 23511
Proximity Label-MS Homo sapiens
129 RPA2 6118
Proximity Label-MS Homo sapiens
130 APC  
Proximity Label-MS Homo sapiens
131 DBN1 1627
Affinity Capture-MS Homo sapiens
132 DTNA  
Proximity Label-MS Homo sapiens
133 MRPL50 54534
Co-fractionation Homo sapiens
134 VAMP2 6844
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
135 TBC1D8  
Proximity Label-MS Homo sapiens
136 CCDC102B  
Two-hybrid Homo sapiens
137 VANGL2  
Proximity Label-MS Homo sapiens
138 NME7 29922
Two-hybrid Homo sapiens
139 IQGAP1 8826
Proximity Label-MS Homo sapiens
140 PRC1 9055
Affinity Capture-MS Homo sapiens
141 GPRC5B 51704
Affinity Capture-MS Homo sapiens
142 OBSL1 23363
Affinity Capture-MS Homo sapiens
143 C1qbp 12261
Affinity Capture-MS Mus musculus
144 Myh9 17886
Affinity Capture-MS Mus musculus
145 TMEM192 201931
Affinity Capture-MS Homo sapiens
146 PTPN14 5784
Proximity Label-MS Homo sapiens
147 PALM 5064
Proximity Label-MS Homo sapiens
148 LHX4  
Affinity Capture-MS Homo sapiens
149 SOX5 6660
Affinity Capture-MS Homo sapiens
150 Erh  
Affinity Capture-MS Mus musculus
151 STAM2 10254
Proximity Label-MS Homo sapiens
152 JMJD6 23210
Affinity Capture-MS Homo sapiens
153 SLC25A10 1468
Proximity Label-MS Homo sapiens
154 SLC1A5 6510
Proximity Label-MS Homo sapiens
155 CEP55 55165
Proximity Label-MS Homo sapiens
156 POLR3K  
Affinity Capture-MS Homo sapiens
157 RAI14 26064
Proximity Label-MS Homo sapiens
158 CBL 867
Reconstituted Complex Homo sapiens
159 ADD2 119
Proximity Label-MS Homo sapiens
160 TUBA1C 84790
Co-fractionation Homo sapiens
161 SIPA1L2  
Proximity Label-MS Homo sapiens
162 KCNB2  
Proximity Label-MS Homo sapiens
163 RAB2A 5862
Affinity Capture-MS Homo sapiens
164 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
165 SLC12A4 6560
Co-fractionation Homo sapiens
166 UBXN6 80700
Affinity Capture-MS Homo sapiens
167 TANC1 85461
Proximity Label-MS Homo sapiens
168 MINK1 50488
Proximity Label-MS Homo sapiens
169 PTOV1  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-localization Homo sapiens
170 DIP2B 57609
Proximity Label-MS Homo sapiens
171 RAB7A 7879
Affinity Capture-MS Homo sapiens
172 SOX6  
Affinity Capture-MS Homo sapiens
173 Rmdn3  
Affinity Capture-MS Mus musculus
174 TJP1 7082
Proximity Label-MS Homo sapiens
175 RICTOR 253260
Proximity Label-MS Homo sapiens
176 USP54 159195
Proximity Label-MS Homo sapiens
177 EGFR 1956
Proximity Label-MS Homo sapiens
178 FAM90A1  
Two-hybrid Homo sapiens
179 FTH1 2495
Affinity Capture-MS Homo sapiens
180 SPTBN1 6711
Proximity Label-MS Homo sapiens
181 GAB1  
Proximity Label-MS Homo sapiens
182 PLEKHA5 54477
Proximity Label-MS Homo sapiens
183 SGTB  
Affinity Capture-MS Homo sapiens
184 SLC3A2 6520
Proximity Label-MS Homo sapiens
185 PKP4 8502
Proximity Label-MS Homo sapiens
186 SLC6A15 55117
Proximity Label-MS Homo sapiens
187 MYEF2 50804
Affinity Capture-MS Homo sapiens
188 CCDC88A 55704
Proximity Label-MS Homo sapiens
189 CPNE8 144402
Proximity Label-MS Homo sapiens
190 RAB31 11031
Co-fractionation Homo sapiens
191 MCUR1 63933
Proximity Label-MS Homo sapiens
192 MARK3 4140
Proximity Label-MS Homo sapiens
193 SLC38A2 54407
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
194 KIF14 9928
Affinity Capture-MS Homo sapiens
195 TCIRG1 10312
Affinity Capture-MS Homo sapiens
196 XPR1 9213
Proximity Label-MS Homo sapiens
197 CAPZA2 830
Affinity Capture-MS Homo sapiens
198 SQSTM1 8878
Proximity Label-MS Homo sapiens
199 Myh10 77579
Affinity Capture-MS Mus musculus
200 MMGT1 93380
Affinity Capture-MS Homo sapiens
201 SLC30A1 7779
Proximity Label-MS Homo sapiens
202 BCL2L1 598
Affinity Capture-MS Homo sapiens
203 KIAA0754  
Proximity Label-MS Homo sapiens
204 NF2 4771
Proximity Label-MS Homo sapiens
205 TUBD1  
Affinity Capture-MS Homo sapiens
206 YKT6 10652
Proximity Label-MS Homo sapiens
207 ANK3  
Proximity Label-MS Homo sapiens
208 TMEM53  
Affinity Capture-MS Homo sapiens
209 SNTB2 6645
Proximity Label-MS Homo sapiens
210 NGB  
Affinity Capture-Western Homo sapiens
211 MAGI1  
Proximity Label-MS Homo sapiens
212 DLG5 9231
Proximity Label-MS Homo sapiens
213 ZDHHC5 25921
Proximity Label-MS Homo sapiens
214 ANK2 287
Proximity Label-MS Homo sapiens
215 OCLN 100506658
Proximity Label-MS Homo sapiens
216 STAT5A 6776
Affinity Capture-MS Homo sapiens
217 EFR3A 23167
Proximity Label-MS Homo sapiens
218 PARD3 56288
Proximity Label-MS Homo sapiens
219 GMIP  
Affinity Capture-MS Homo sapiens
220 EPB41L5 57669
Proximity Label-MS Homo sapiens
221 Chmp4b 75608
Affinity Capture-MS Mus musculus
222 ADGRL2 23266
Proximity Label-MS Homo sapiens
223 ADD3 120
Proximity Label-MS Homo sapiens
224 PTPN1 5770
Affinity Capture-MS Homo sapiens
225 Actb 11461
Affinity Capture-MS Mus musculus
226 PI4KA 5297
Proximity Label-MS Homo sapiens
227 PCGF2 7703
Affinity Capture-MS Homo sapiens
228 GNG12 55970
Co-fractionation Homo sapiens
229 RUVBL1 8607
Proximity Label-MS Homo sapiens
230 PAK4 10298
Proximity Label-MS Homo sapiens
231 FAM171A2 284069
Proximity Label-MS Homo sapiens
232 NUMBL 9253
Proximity Label-MS Homo sapiens
233 DSC2 1824
Proximity Label-MS Homo sapiens
234 ETV4  
Affinity Capture-MS Homo sapiens
235 PHLDB2 90102
Proximity Label-MS Homo sapiens
236 SLC19A1 6573
Proximity Label-MS Homo sapiens
237 SNAP23 8773
Proximity Label-MS Homo sapiens
238 TFCP2 7024
Affinity Capture-MS Homo sapiens
239 ESYT2 57488
Proximity Label-MS Homo sapiens
240 GPRIN1 114787
Proximity Label-MS Homo sapiens
241 PLEKHA1 59338
Proximity Label-MS Homo sapiens
242 EPB41L3 23136
Proximity Label-MS Homo sapiens
243 KIAA1217 56243
Proximity Label-MS Homo sapiens
244 BANP  
Two-hybrid Homo sapiens
245 IRS4 8471
Proximity Label-MS Homo sapiens
246 ATP5A1 498
Co-fractionation Homo sapiens
247 AKAP12 9590
Proximity Label-MS Homo sapiens
248 PARK2  
Affinity Capture-MS Homo sapiens
249 CXADR 1525
Proximity Label-MS Homo sapiens
250 DIABLO 56616
Proximity Label-MS Homo sapiens
251 CHMP4B 128866
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
252 NUMB 8650
Proximity Label-MS Homo sapiens
253 U2AF2 11338
Affinity Capture-MS Homo sapiens
254 PAX8  
Affinity Capture-MS Homo sapiens
255 SMAD2 4087
Affinity Capture-MS Homo sapiens
256 PIK3R1 5295
Proximity Label-MS Homo sapiens
257 ARHGEF12 23365
Proximity Label-MS Homo sapiens
258 ARL13B 200894
Proximity Label-MS Homo sapiens
259 CA9 768
Co-fractionation Homo sapiens
260 SLC4A7 9497
Proximity Label-MS Homo sapiens
261 Rab5c 19345
Affinity Capture-MS Mus musculus
262 ACAT1 38
Co-fractionation Homo sapiens
263 ECT2 1894
Affinity Capture-MS Homo sapiens
264 FERMT2 10979
Proximity Label-MS Homo sapiens
265 PDGFRA 5156
Affinity Capture-MS Homo sapiens
266 SLC34A2 10568
Affinity Capture-MS Homo sapiens
267 FMN2  
Proximity Label-MS Homo sapiens
268 OCIAD1 54940
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
269 CDC42BPA 8476
Proximity Label-MS Homo sapiens
270 ARHGAP40  
Affinity Capture-MS Homo sapiens
271 SYNPO 11346
Affinity Capture-MS Homo sapiens
272 MYO1D 4642
Proximity Label-MS Homo sapiens
273 Flnb 286940
Affinity Capture-MS Mus musculus
274 PTPN13 5783
Proximity Label-MS Homo sapiens
275 EPB41 2035
Proximity Label-MS Homo sapiens
276 RAF1 5894
Proximity Label-MS Homo sapiens
277 RANBP1 5902
Affinity Capture-MS Homo sapiens
278 KIAA0368 23392
Affinity Capture-Western Homo sapiens
279 HAX1  
Proximity Label-MS Homo sapiens
280 MRPL38  
Co-fractionation Homo sapiens
281 CASKIN2  
Proximity Label-MS Homo sapiens
282 SLC14A1  
Affinity Capture-MS Homo sapiens
283 TUBB2A 7280
Proximity Label-MS Homo sapiens
284 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
285 PVRL2 5819
Proximity Label-MS Homo sapiens
286 ITSN1 6453
Proximity Label-MS Homo sapiens
287 JUP 3728
Proximity Label-MS Homo sapiens
288 SPTBN2 6712
Proximity Label-MS Homo sapiens
289 ICT1 3396
Co-fractionation Homo sapiens
290 FAM171B  
Proximity Label-MS Homo sapiens
291 LSR 51599
Proximity Label-MS Homo sapiens
292 PDHA1 5160
Affinity Capture-MS Homo sapiens
293 EFNB1 1947
Proximity Label-MS Homo sapiens
294 ERLIN2 11160
Co-fractionation Homo sapiens
295 LAMTOR2 28956
Co-fractionation Homo sapiens
296 ZDHHC23  
Affinity Capture-MS Homo sapiens
297 DDX58 23586
Affinity Capture-RNA Homo sapiens
298 NF1 4763
Proximity Label-MS Homo sapiens
299 AFG3L2 10939
Proximity Label-MS Homo sapiens
300 PPP1R13B  
Proximity Label-MS Homo sapiens
301 FRS2 10818
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
302 FLNA 2316
Affinity Capture-MS Homo sapiens
303 KANK2 25959
Proximity Label-MS Homo sapiens
304 SERBP1 26135
Affinity Capture-MS Homo sapiens
305 AR 367
Affinity Capture-MS Homo sapiens
306 VIM 7431
Affinity Capture-MS Homo sapiens
307 CAV1 857
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-localization Homo sapiens
Proximity Label-MS Homo sapiens
308 NOTCH1 4851
Proximity Label-MS Homo sapiens
309 ABLIM1 3983
Proximity Label-MS Homo sapiens
310 MAEA 10296
Co-fractionation Homo sapiens
311 UBA52 7311
Proximity Label-MS Homo sapiens
312 POLR2B 5431
Proximity Label-MS Homo sapiens
313 ARHGAP21 57584
Proximity Label-MS Homo sapiens
314 TPGS1  
Proximity Label-MS Homo sapiens
315 CDC42BPB 9578
Proximity Label-MS Homo sapiens
316 CEP89 84902
Proximity Label-MS Homo sapiens
317 VAPA 9218
Affinity Capture-MS Homo sapiens
318 WWP1 11059
Affinity Capture-MS Homo sapiens
319 MAN2A1 4124
Co-fractionation Homo sapiens
320 TRIP13 9319
Proximity Label-MS Homo sapiens
321 RING1 6015
Affinity Capture-MS Homo sapiens
322 RPL36A-HNRNPH2 100529097
Proximity Label-MS Homo sapiens
323 CCT4 10575
Proximity Label-MS Homo sapiens
324 BASP1 10409
Proximity Label-MS Homo sapiens
325 INCENP 3619
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
326 SNX6 58533
Proximity Label-MS Homo sapiens
327 SLC12A2 6558
Proximity Label-MS Homo sapiens
328 HSPA5 3309
Cross-Linking-MS (XL-MS) Homo sapiens
329 YES1 7525
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
330 MARK2 2011
Proximity Label-MS Homo sapiens
331 Trim69  
Affinity Capture-MS Mus musculus
332 SLC39A10 57181
Proximity Label-MS Homo sapiens
333 SCRIB 23513
Proximity Label-MS Homo sapiens
334 MRPS5 64969
Co-fractionation Homo sapiens
335 HGS 9146
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
336 ADD1 118
Proximity Label-MS Homo sapiens
337 TIAM1  
Proximity Label-MS Homo sapiens
338 ABCE1 6059
Affinity Capture-MS Homo sapiens
339 SURF1  
Proximity Label-MS Homo sapiens
340 MYO1B 4430
Proximity Label-MS Homo sapiens
341 PEAK1 79834
Proximity Label-MS Homo sapiens
342 EED  
Affinity Capture-MS Homo sapiens
343 VPS53 55275
Proximity Label-MS Homo sapiens
344 INADL 10207
Proximity Label-MS Homo sapiens
345 ERBB2 2064
Proximity Label-MS Homo sapiens
346 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
347 TMEM63B 55362
Affinity Capture-MS Homo sapiens
348 CASK 8573
Proximity Label-MS Homo sapiens
349 APOO 79135
Proximity Label-MS Homo sapiens
350 CDCA3 83461
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
351 POLD1 5424
Affinity Capture-MS Homo sapiens
352 COX15 1355
Affinity Capture-MS Homo sapiens
353 PSMD4 5710
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
354 TNKS1BP1 85456
Proximity Label-MS Homo sapiens
355 CNNM3 26505
Proximity Label-MS Homo sapiens
356 C19orf26 255057
Proximity Label-MS Homo sapiens
357 CCDC85C  
Proximity Label-MS Homo sapiens
358 FAM135A  
Proximity Label-MS Homo sapiens
359 RAB11FIP5 26056
Proximity Label-MS Homo sapiens
360 ANKRD26 22852
Proximity Label-MS Homo sapiens
361 CTNNAL1 8727
Proximity Label-MS Homo sapiens
362 LIMA1 51474
Affinity Capture-MS Homo sapiens
363 SLC25A24 29957
Co-fractionation Homo sapiens
364 COX5B 1329
Two-hybrid Homo sapiens
365 FANCD2  
Affinity Capture-MS Homo sapiens
366 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
367 SLC38A1 81539
Proximity Label-MS Homo sapiens
368 ATP2B1 490
Proximity Label-MS Homo sapiens
369 CTNNB1 1499
Proximity Label-MS Homo sapiens
370 Bmpr1a  
Affinity Capture-MS Mus musculus
371 FAM171A1 221061
Proximity Label-MS Homo sapiens
372 TRIO 7204
Proximity Label-MS Homo sapiens
373 SLC7A5 8140
Proximity Label-MS Homo sapiens
374 EHBP1 23301
Proximity Label-MS Homo sapiens
375 Vps4b 20479
Affinity Capture-MS Mus musculus
376 AURKB 9212
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
377 LZTS2 84445
Proximity Label-MS Homo sapiens
378 KIRREL 55243
Proximity Label-MS Homo sapiens
379 NIN 51199
Proximity Label-MS Homo sapiens
380 IRF8  
Affinity Capture-MS Homo sapiens
381 AKAP5  
Proximity Label-MS Homo sapiens
382 ELF2  
Affinity Capture-MS Homo sapiens
383 PPFIBP1 8496
Proximity Label-MS Homo sapiens
384 PPFIA1 8500
Proximity Label-MS Homo sapiens
385 IRF3 3661
Affinity Capture-MS Homo sapiens
386 FARP1 10160
Proximity Label-MS Homo sapiens
387 KIAA1522 57648
Proximity Label-MS Homo sapiens
388 Cbx1  
Affinity Capture-MS Mus musculus
389 KIAA1549  
Proximity Label-MS Homo sapiens
390 GLUD1 2746
Proximity Label-MS Homo sapiens
391 SLC25A11 8402
Co-fractionation Homo sapiens
392 SPTAN1 6709
Proximity Label-MS Homo sapiens
393 Tmod3 50875
Affinity Capture-MS Mus musculus
394 ROCK1 6093
Proximity Label-MS Homo sapiens
395 ELF5  
Affinity Capture-MS Homo sapiens
396 DNAJC19 131118
Proximity Label-MS Homo sapiens
397 DNAJA2 10294
Proximity Label-MS Homo sapiens
398 MRPS31  
Co-fractionation Homo sapiens
399 FAM3C 10447
Co-fractionation Homo sapiens
400 RPS15 6209
Co-fractionation Homo sapiens
401 NISCH 11188
Proximity Label-MS Homo sapiens
402 EPB41L2 2037
Proximity Label-MS Homo sapiens
403 ROR2 4920
Proximity Label-MS Homo sapiens
404 STXBP4 252983
Proximity Label-MS Homo sapiens
405 SLC25A4 291
Proximity Label-MS Homo sapiens
406 CAD 790
Proximity Label-MS Homo sapiens
407 TRIM31  
Affinity Capture-MS Homo sapiens
408 SLC6A8 6535
Proximity Label-MS Homo sapiens
409 PIK3R2 5296
Proximity Label-MS Homo sapiens
410 Ptpn23 104831
Affinity Capture-MS Mus musculus
411 LRRC49  
Proximity Label-MS Homo sapiens
412 CAPN10  
Two-hybrid Homo sapiens
413 DSG2 1829
Proximity Label-MS Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here