Gene description for Rpl11
Gene name ribosomal protein L11
Gene symbol Rpl11
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rpl11
ExoCarta ExoCarta_362631
Vesiclepedia VP_362631
Entrez Gene 362631
UniProt P62914  
 Rpl11 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Rpl11
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IEA
    structural constituent of ribosome GO:0003735 ISO
    5S rRNA binding GO:0008097 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    peroxisome proliferator activated receptor binding GO:0042975 IPI
    ubiquitin ligase inhibitor activity GO:1990948 ISO
Biological Process
    ribosomal large subunit assembly GO:0000027 ISO
    cytoplasmic translation GO:0002181 ISO
    rRNA processing GO:0006364 ISO
    translation GO:0006412 IBA
    translation GO:0006412 IEA
    protein targeting GO:0006605 ISO
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISS
    protein localization to nucleus GO:0034504 ISO
    protein localization to nucleus GO:0034504 ISS
    ribosomal large subunit biogenesis GO:0042273 ISO
    negative regulation of DNA-binding transcription factor activity GO:0043433 IDA
    protein stabilization GO:0050821 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
    regulation of signal transduction by p53 class mediator GO:1901796 ISO
    positive regulation of signal transduction by p53 class mediator GO:1901798 ISO
    positive regulation of signal transduction by p53 class mediator GO:1901798 ISS
    negative regulation of ubiquitin protein ligase activity GO:1904667 ISO
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 ISO
    negative regulation of protein neddylation GO:2000435 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    cytoplasm GO:0005737 ISO
    ribosome GO:0005840 IEA
    ribosome GO:0005840 NAS
    large ribosomal subunit GO:0015934 ISO
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 IDA
    cytosolic ribosome GO:0022626 ISO
    protein-containing complex GO:0032991 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    cytoplasmic side of rough endoplasmic reticulum membrane GO:0098556 ISO
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified Rpl11 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Rpl11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snca  
Affinity Capture-MS Rattus norvegicus
2 Sumo3  
Affinity Capture-MS Rattus norvegicus
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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