Gene description for Clca4a
Gene name chloride channel calcium activated 4A
Gene symbol Clca4a
Other names/aliases 9130020L07Rik
AI504701
Clca4
Clca6
Species Mus musculus
 Database cross references - Clca4a
ExoCarta ExoCarta_99663
Vesiclepedia VP_99663
Entrez Gene 99663
 Clca4a identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Clca4a
Molecular Function
    intracellularly calcium-gated chloride channel activity GO:0005229 IBA
    intracellularly calcium-gated chloride channel activity GO:0005229 IEA
    chloride channel activity GO:0005254 IDA
    chloride channel activity GO:0005254 IMP
    metallopeptidase activity GO:0008237 IEA
    ligand-gated monoatomic ion channel activity GO:0015276 IMP
    metal ion binding GO:0046872 IEA
Biological Process
    proteolysis GO:0006508 IEA
    chloride transport GO:0006821 IMP
    chloride transmembrane transport GO:1902476 IEA
Subcellular Localization
    extracellular region GO:0005576 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    apical plasma membrane GO:0016324 IEA
 Experiment description of studies that identified Clca4a in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Clca4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Clca4a is involved
No pathways found





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