Gene description for HEPH
Gene name hephaestin
Gene symbol HEPH
Other names/aliases CPL
Species Homo sapiens
 Database cross references - HEPH
ExoCarta ExoCarta_9843
Entrez Gene 9843
HGNC 4866
MIM 300167
UniProt Q9BQS7  
 HEPH identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
 Gene ontology annotations for HEPH
Molecular Function
    ferroxidase activity GO:0004322 IMP
    copper ion binding GO:0005507 IMP
    ferrous iron binding GO:0008198 IMP
Biological Process
    oxidation-reduction process GO:0055114 IEA
    iron ion transport GO:0006826 IMP
    copper ion transport GO:0006825 IEA
    transmembrane transport GO:0055085 TAS
    cellular iron ion homeostasis GO:0006879 TAS
Subcellular Localization
    intracellular GO:0005622 IDA
    basolateral plasma membrane GO:0016323 IDA
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified HEPH in exosomes
1
Experiment ID 21
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for HEPH
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which HEPH is involved
PathwayEvidenceSource
Iron uptake and transport TAS Reactome
Metal ion SLC transporters TAS Reactome





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