Experiment description of studies that identified cirA in sEVs

1 Experiment ID 576 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain BL21 (ClearColi) - 18h culture Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

2 Experiment ID 577 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain BL21 (ClearColi) - 21h culture Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

3 Experiment ID 578 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain BL21 (ClearColi) - 24h culture Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

4 Experiment ID 579 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain BL21 (ClearColi) - 27h culture Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

5 Experiment ID 583 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 5 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

6 Experiment ID 584 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 6 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

7 Experiment ID 585 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 7 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

8 Experiment ID 586 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 8 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

9 Experiment ID 587 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 9 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

10 Experiment ID 588 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 10 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

11 Experiment ID 589 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 11 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

12 Experiment ID 590 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 12 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

13 Experiment ID 591 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

14 Experiment ID 592 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

15 Experiment ID 593 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

16 Experiment ID 594 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

17 Experiment ID 596 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

18 Experiment ID 597 MISEV standards ✘ Biophysical techniques Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 36648227     Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M." Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

Protein-protein interactions for cirA

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which cirA is involved

No pathways found