Gene description for Spock2
Gene name sparc/osteonectin, cwcv and kazal-like domains proteoglycan 2
Gene symbol Spock2
Other names/aliases AA407235
Gcap26
mKIAA0275
Species Mus musculus
 Database cross references - Spock2
ExoCarta ExoCarta_94214
Vesiclepedia VP_94214
Entrez Gene 94214
UniProt Q9ER58  
 Spock2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Spock2
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    collagen binding GO:0005518 IBA
    glycosaminoglycan binding GO:0005539 IDA
    extracellular matrix structural constituent conferring compression resistance GO:0030021 RCA
    extracellular matrix binding GO:0050840 IBA
    extracellular matrix binding GO:0050840 IDA
Biological Process
    positive regulation of cell-substrate adhesion GO:0010811 IDA
    extracellular matrix organization GO:0030198 IDA
    cellular response to leukemia inhibitory factor GO:1990830 IEP
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 IBA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Spock2 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Spock2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Spock2 is involved
No pathways found





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