Gene description for Dock2
Gene name dedicator of cyto-kinesis 2
Gene symbol Dock2
Other names/aliases AI662014
AW122239
CED-5
Hch
MBC
Species Mus musculus
 Database cross references - Dock2
ExoCarta ExoCarta_94176
Vesiclepedia VP_94176
Entrez Gene 94176
UniProt Q8C3J5  
 Dock2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dock2
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 ISO
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    GTPase activator activity GO:0005096 IMP
    small GTPase binding GO:0031267 IBA
    T cell receptor binding GO:0042608 ISO
    T cell receptor binding GO:0042608 ISS
Biological Process
    membrane raft polarization GO:0001766 IMP
    establishment of T cell polarity GO:0001768 IMP
    immunological synapse formation GO:0001771 IMP
    myeloid dendritic cell activation involved in immune response GO:0002277 IMP
    chemotaxis GO:0006935 IMP
    cytoskeleton organization GO:0007010 IDA
    small GTPase-mediated signal transduction GO:0007264 IEA
    myoblast fusion GO:0007520 IBA
    cell migration GO:0016477 IBA
    actin cytoskeleton organization GO:0030036 IEA
    positive regulation of Rac protein signal transduction GO:0035022 IC
    T cell proliferation GO:0042098 IMP
    T cell activation GO:0042110 IMP
    macropinocytosis GO:0044351 IMP
    positive thymic T cell selection GO:0045059 IMP
    negative thymic T cell selection GO:0045060 IMP
    alpha-beta T cell activation GO:0046631 IMP
    alpha-beta T cell proliferation GO:0046633 IMP
    positive regulation of phagocytosis GO:0050766 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IBA
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 IEA
 Experiment description of studies that identified Dock2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dock2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Pkd2  
Co-fractionation Mus musculus
View the network image/svg+xml



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