Experiment description of studies that identified Nap1l1 in sEVs

1 Experiment ID 225 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25998041     Organism Rattus norvegicus Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats. Authors "Lee JE, Moon PG, Lee IK, Baek MC" Journal name Protein J Publication year 2015 Sample Adipocytes Sample name Adipocytes Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 90 MISEV standards ✔ EM Biophysical techniques ✔ HSC70|HSP90|TSG101|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E" Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

3 Experiment ID 94 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

4 Experiment ID 95 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

5 Experiment ID 101 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP90|HSC70|MHCI Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [QSTAR] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

Protein-protein interactions for Nap1l1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Mib1   Affinity Capture-MS Rattus norvegicus View the network

Pathways in which Nap1l1 is involved

No pathways found