Gene description for MGAM
Gene name maltase-glucoamylase
Gene symbol MGAM
Other names/aliases MG
MGA
Species Homo sapiens
 Database cross references - MGAM
ExoCarta ExoCarta_8972
Vesiclepedia VP_8972
Entrez Gene 8972
HGNC 7043
MIM 154360
UniProt O43451  
 MGAM identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Urine 15326289    
Urine 22418980    
Urine 22418980    
Urine 25452312    
 Gene ontology annotations for MGAM
Molecular Function
    catalytic activity GO:0003824 TAS
    glucan 1,4-alpha-glucosidase activity GO:0004339 IEA
    alpha-1,4-glucosidase activity GO:0004558 IBA
    alpha-1,4-glucosidase activity GO:0004558 IDA
    oligo-1,6-glucosidase activity GO:0004574 IEA
    protein binding GO:0005515 IPI
    amylase activity GO:0016160 IEA
    carbohydrate binding GO:0030246 IEA
Biological Process
    maltose catabolic process GO:0000025 IDA
    starch catabolic process GO:0005983 TAS
    dextrin catabolic process GO:1901027 IDA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    apical plasma membrane GO:0016324 IDA
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified MGAM in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
5
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 238
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors "Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R."
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MGAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CPLX4  
Two-hybrid Homo sapiens
2 CREB3L1  
Two-hybrid Homo sapiens
3 TMEM14B  
Two-hybrid Homo sapiens
4 NCF1  
Two-hybrid Homo sapiens
5 GPR42  
Two-hybrid Homo sapiens
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