Gene description for Rala
Gene name v-ral simian leukemia viral oncogene homolog A (ras related)
Gene symbol Rala
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rala
ExoCarta ExoCarta_81757
Vesiclepedia VP_81757
Entrez Gene 81757
UniProt P63322  
 Rala identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Rala
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 NAS
    myosin binding GO:0017022 ISO
    GDP binding GO:0019003 IBA
    GDP binding GO:0019003 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    Edg-2 lysophosphatidic acid receptor binding GO:0031755 ISO
    Edg-2 lysophosphatidic acid receptor binding GO:0031755 ISS
    ATPase binding GO:0051117 ISO
Biological Process
    neural tube closure GO:0001843 ISO
    exocytosis GO:0006887 IEA
    signal transduction GO:0007165 IEA
    Ras protein signal transduction GO:0007265 IBA
    Ras protein signal transduction GO:0007265 ISO
    regulation of exocytosis GO:0017157 IBA
    regulation of exocytosis GO:0017157 ISO
    regulation of exocytosis GO:0017157 ISS
    receptor internalization GO:0031623 ISO
    regulation of actin cytoskeleton organization GO:0032956 ISO
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 ISO
    cell division GO:0051301 IEA
    positive regulation of filopodium assembly GO:0051491 ISO
    membrane raft localization GO:0051665 ISO
    membrane raft localization GO:0051665 ISS
    establishment of protein localization to mitochondrion GO:0072655 ISO
    establishment of protein localization to mitochondrion GO:0072655 ISS
    positive regulation of mitochondrial fission GO:0090141 ISO
    positive regulation of mitochondrial fission GO:0090141 ISS
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 EXP
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IDA
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IMP
Subcellular Localization
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    cell surface GO:0009986 ISO
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 IEA
    endocytic vesicle GO:0030139 ISO
    cleavage furrow GO:0032154 ISO
    cleavage furrow GO:0032154 ISS
    Flemming body GO:0090543 IEA
    synaptic membrane GO:0097060 IDA
    Schaffer collateral - CA1 synapse GO:0098685 EXP
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
 Experiment description of studies that identified Rala in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rala
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ybx3  
Two-hybrid Rattus norvegicus
2 Ralbp1  
Two-hybrid Rattus norvegicus
3 Mib1  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Rala is involved
No pathways found





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