Gene description for Rab13
Gene name RAB13, member RAS oncogene family
Gene symbol Rab13
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rab13
ExoCarta ExoCarta_81756
Vesiclepedia VP_81756
Entrez Gene 81756
UniProt P35286  
 Rab13 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Rab13
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    protein kinase A catalytic subunit binding GO:0034236 IPI
Biological Process
    exocytosis GO:0006887 IBA
    Golgi to plasma membrane transport GO:0006893 IEA
    Golgi to plasma membrane transport GO:0006893 ISO
    protein kinase A signaling GO:0010737 IBA
    protein kinase A signaling GO:0010737 IEA
    protein kinase A signaling GO:0010737 IMP
    protein kinase A signaling GO:0010737 ISO
    protein transport GO:0015031 IEA
    endosomal transport GO:0016197 ISO
    endosomal transport GO:0016197 ISS
    cortical actin cytoskeleton organization GO:0030866 IMP
    neuron projection development GO:0031175 IEA
    neuron projection development GO:0031175 IMP
    neuron projection development GO:0031175 ISO
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 IEA
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    cellular response to insulin stimulus GO:0032869 IMP
    endothelial cell chemotaxis GO:0035767 IEA
    endothelial cell chemotaxis GO:0035767 ISO
    endothelial cell chemotaxis GO:0035767 ISS
    glucose homeostasis GO:0042593 IC
    trans-Golgi network to recycling endosome transport GO:0044795 ISO
    trans-Golgi network to recycling endosome transport GO:0044795 ISS
    positive regulation of D-glucose import GO:0046326 IC
    bicellular tight junction assembly GO:0070830 IEA
    bicellular tight junction assembly GO:0070830 ISO
    bicellular tight junction assembly GO:0070830 ISS
    protein localization to plasma membrane GO:0072659 IDA
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    protein localization to plasma membrane GO:0072659 ISS
    establishment of Sertoli cell barrier GO:0097368 IMP
    protein localization to cell leading edge GO:1902463 IEA
    protein localization to cell leading edge GO:1902463 ISO
    protein localization to cell leading edge GO:1902463 ISS
Subcellular Localization
    endosome GO:0005768 IBA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    trans-Golgi network GO:0005802 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell-cell junction GO:0005911 ISO
    bicellular tight junction GO:0005923 IEA
    bicellular tight junction GO:0005923 ISO
    bicellular tight junction GO:0005923 ISS
    synaptic vesicle GO:0008021 IBA
    lateral plasma membrane GO:0016328 IEA
    lateral plasma membrane GO:0016328 ISO
    lateral plasma membrane GO:0016328 ISS
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    lamellipodium GO:0030027 ISS
    endocytic vesicle GO:0030139 IEA
    endocytic vesicle GO:0030139 ISO
    endocytic vesicle GO:0030139 ISS
    trans-Golgi network transport vesicle GO:0030140 IBA
    cytoplasmic vesicle membrane GO:0030659 TAS
    cytoplasmic vesicle GO:0031410 ISO
    insulin-responsive compartment GO:0032593 IDA
    neuron projection GO:0043005 IEA
    neuron projection GO:0043005 ISO
    neuron projection GO:0043005 ISS
    recycling endosome GO:0055037 ISO
    recycling endosome GO:0055037 ISS
    recycling endosome membrane GO:0055038 IEA
 Experiment description of studies that identified Rab13 in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rab13
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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