Gene description for VMP1
Gene name vacuole membrane protein 1
Gene symbol VMP1
Other names/aliases EPG3
TANGO5
TMEM49
Species Homo sapiens
 Database cross references - VMP1
ExoCarta ExoCarta_81671
Vesiclepedia VP_81671
Entrez Gene 81671
HGNC 29559
MIM 611753
UniProt Q96GC9  
 VMP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for VMP1
Molecular Function
    protein binding GO:0005515 IPI
    phospholipid scramblase activity GO:0017128 IDA
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 IDA
    autophagosome assembly GO:0000045 IMP
    autophagy GO:0006914 IDA
    Golgi organization GO:0007030 IBA
    embryo implantation GO:0007566 IEA
    autophagosome membrane docking GO:0016240 IDA
    plasma membrane phospholipid scrambling GO:0017121 IEA
    cell junction assembly GO:0034329 IMP
    lipoprotein transport GO:0042953 IMP
    cell-cell adhesion GO:0098609 IMP
    organelle localization by membrane tethering GO:0140056 IDA
    positive regulation of ATPase-coupled calcium transmembrane transporter activity GO:1901896 IDA
    mitochondrion-endoplasmic reticulum membrane tethering GO:1990456 IDA
Subcellular Localization
    phagophore assembly site GO:0000407 IEA
    autophagosome membrane GO:0000421 ISS
    nucleolus GO:0005730 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    plasma membrane GO:0005886 IEA
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 IBA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
 Experiment description of studies that identified VMP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for VMP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDH13 1012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MAGEA8  
Affinity Capture-MS Homo sapiens
3 ERGIC3 51614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 EDEM1  
Affinity Capture-MS Homo sapiens
5 HSD17B11 51170
Proximity Label-MS Homo sapiens
6 TJP1 7082
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
7 FCGR3B  
Affinity Capture-MS Homo sapiens
8 RIT1 6016
Negative Genetic Homo sapiens
9 PTGIR  
Affinity Capture-MS Homo sapiens
10 ART3  
Affinity Capture-MS Homo sapiens
11 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 REEP5 7905
Proximity Label-MS Homo sapiens
13 FAM20C 56975
Affinity Capture-MS Homo sapiens
14 MAS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CLEC2D  
Affinity Capture-MS Homo sapiens
16 EGFR 1956
Negative Genetic Homo sapiens
17 METTL7A 25840
Proximity Label-MS Homo sapiens
18 LAMP3  
Proximity Label-MS Homo sapiens
19 LRRC59 55379
Proximity Label-MS Homo sapiens
20 LMNB1 4001
Proximity Label-MS Homo sapiens
21 NUP155 9631
Proximity Label-MS Homo sapiens
22 LPAR6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PTH2R  
Affinity Capture-MS Homo sapiens
24 CD70 970
Affinity Capture-MS Homo sapiens
25 MEX3A  
Affinity Capture-RNA Homo sapiens
26 PANX1 24145
Proximity Label-MS Homo sapiens
27 KIAA1429 25962
Affinity Capture-MS Homo sapiens
28 BCAP31 10134
Proximity Label-MS Homo sapiens
29 HSD3B7 80270
Proximity Label-MS Homo sapiens
30 STIM1 6786
Proximity Label-MS Homo sapiens
31 GJD3  
Proximity Label-MS Homo sapiens
32 EMD 2010
Proximity Label-MS Homo sapiens
33 LAMP2 3920
Proximity Label-MS Homo sapiens
34 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
35 LRRC25  
Affinity Capture-MS Homo sapiens
36 CLPP 8192
Proximity Label-MS Homo sapiens
37 DPEP1 1800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MFSD4  
Affinity Capture-MS Homo sapiens
39 ERGIC1 57222
Proximity Label-MS Homo sapiens
40 GLMP 112770
Affinity Capture-MS Homo sapiens
41 KLRC4  
Affinity Capture-MS Homo sapiens
42 KLK11 11012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 LPCAT2 54947
Affinity Capture-MS Homo sapiens
44 CACNG6  
Affinity Capture-MS Homo sapiens
45 B3GAT1  
Proximity Label-MS Homo sapiens
46 NMUR2  
Affinity Capture-MS Homo sapiens
47 SEC62 7095
Proximity Label-MS Homo sapiens
48 ATP2A1 487
Proximity Label-MS Homo sapiens
49 SPPL2B 56928
Affinity Capture-MS Homo sapiens
50 GPR45  
Affinity Capture-MS Homo sapiens
51 CMTM5  
Affinity Capture-MS Homo sapiens
52 MTOR 2475
Affinity Capture-MS Homo sapiens
53 FAM174A 345757
Affinity Capture-MS Homo sapiens
54 TMEM9 252839
Affinity Capture-MS Homo sapiens
55 RAB7A 7879
Proximity Label-MS Homo sapiens
56 SLC1A1 6505
Affinity Capture-MS Homo sapiens
57 HGS 9146
Reconstituted Complex Homo sapiens
58 RPN1 6184
Proximity Label-MS Homo sapiens
59 KRAS 3845
Negative Genetic Homo sapiens
60 RAB5C 5878
Proximity Label-MS Homo sapiens
61 KLRC1  
Affinity Capture-MS Homo sapiens
62 SSR1 6745
Proximity Label-MS Homo sapiens
63 CKAP4 10970
Proximity Label-MS Homo sapiens
64 P2RY8  
Affinity Capture-MS Homo sapiens
65 SLC22A4 6583
Affinity Capture-MS Homo sapiens
66 ELOVL5 60481
Proximity Label-MS Homo sapiens
67 FPR2  
Affinity Capture-MS Homo sapiens
68 SEC61B 10952
Proximity Label-MS Homo sapiens
69 RPN2 6185
Proximity Label-MS Homo sapiens
70 DERL1 79139
Proximity Label-MS Homo sapiens
71 APLNR  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which VMP1 is involved
No pathways found





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