Gene description for Gna12
Gene name guanine nucleotide binding protein (G protein) alpha 12
Gene symbol Gna12
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gna12
ExoCarta ExoCarta_81663
Vesiclepedia VP_81663
Entrez Gene 81663
UniProt Q63210  
 Gna12 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Gna12
Molecular Function
    GTPase activity GO:0003924 IBA
    G protein activity GO:0003925 ISO
    GTP binding GO:0005525 IEA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    D5 dopamine receptor binding GO:0031752 IBA
    D5 dopamine receptor binding GO:0031752 IPI
    metal ion binding GO:0046872 IEA
    protein phosphatase 2A binding GO:0051721 ISO
    protein phosphatase activator activity GO:0072542 IEA
    protein phosphatase activator activity GO:0072542 ISO
Biological Process
    in utero embryonic development GO:0001701 ISO
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    G protein-coupled dopamine receptor signaling pathway GO:0007212 TAS
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 ISO
    regulation of blood pressure GO:0008217 ISO
    regulation of cell shape GO:0008360 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    regulation of fibroblast migration GO:0010762 ISO
    regulation of fibroblast migration GO:0010762 ISS
    cell differentiation GO:0030154 ISO
    regulation of TOR signaling GO:0032006 ISO
    regulation of TOR signaling GO:0032006 ISS
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 ISO
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 ISS
    intracellular signal transduction GO:0035556 ISO
    embryonic digit morphogenesis GO:0042733 ISO
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 IMP
    negative regulation of vascular associated smooth muscle cell migration GO:1904753 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 TAS
    lateral plasma membrane GO:0016328 ISO
    brush border membrane GO:0031526 IBA
    brush border membrane GO:0031526 IDA
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Gna12 in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gna12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gna12 is involved
PathwayEvidenceSource
G alpha (12/13) signalling events IEA Reactome
GPCR downstream signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome





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