|
Gene description for Anpep |
| Gene name |
alanyl (membrane) aminopeptidase |
| Gene symbol |
Anpep |
| Other names/aliases |
AP-M AP-N Apm Apn KZP Lap1 rAPN |
| Species |
Rattus norvegicus |
Database cross references - Anpep |
| ExoCarta |
ExoCarta_81641 |
| Vesiclepedia |
VP_81641 |
| Entrez Gene |
81641 |
| UniProt |
P15684
|
Anpep identified in sEVs derived from the following tissue/cell type |
|
Hepatocytes
|
19367702
|
|
Reticulocytes
|
21828046
|
|
Reticulocytes
|
21828046
|
|
Reticulocytes
|
21828046
|
|
Urine
|
20535238
|
Gene ontology annotations for Anpep |
|
|
Experiment description of studies that identified Anpep in sEVs |
| 1 |
| Experiment ID |
35 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
TSG101|Alix|CD81|CD63
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
19367702
|
| Organism |
Rattus norvegicus |
| Experiment description |
Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. |
| Authors |
"Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM" |
| Journal name |
JPR
|
| Publication year |
2008 |
| Sample |
Hepatocytes |
| Sample name |
Hepatocytes |
| Isolation/purification methods |
Differential centrifugation Filtration Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] Western blotting |
|
|
| 2 |
| Experiment ID |
96 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP90|HSC70|MHCI
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [Orbitrap]
|
| PubMed ID |
21828046
|
| Organism |
Rattus norvegicus |
| Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
| Authors |
"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." |
| Journal name |
JBC
|
| Publication year |
2011 |
| Sample |
Reticulocytes |
| Sample name |
Reticulocytes - D2 Rep 2 |
| Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
| Flotation density |
1.16-1.21 g/mL
|
| Molecules identified in the study |
Protein Lipids |
| Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
| 3 |
| Experiment ID |
97 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP90|HSC70|MHCI
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [Orbitrap]
|
| PubMed ID |
21828046
|
| Organism |
Rattus norvegicus |
| Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
| Authors |
"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." |
| Journal name |
JBC
|
| Publication year |
2011 |
| Sample |
Reticulocytes |
| Sample name |
Reticulocytes - D4 Rep 2 |
| Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
| Flotation density |
1.16-1.21 g/mL
|
| Molecules identified in the study |
Protein Lipids |
| Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
| 4 |
| Experiment ID |
98 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP90|HSC70|MHCI
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [Orbitrap]
|
| PubMed ID |
21828046
|
| Organism |
Rattus norvegicus |
| Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
| Authors |
"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." |
| Journal name |
JBC
|
| Publication year |
2011 |
| Sample |
Reticulocytes |
| Sample name |
Reticulocytes - D7 Rep 2 |
| Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
| Flotation density |
1.13-1.25 g/mL
|
| Molecules identified in the study |
Protein Lipids |
| Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
| 5 |
| Experiment ID |
192 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
TSG101|HSP70|CD63|CD81|FLOT
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20535238
|
| Organism |
Rattus norvegicus |
| Experiment description |
Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples. |
| Authors |
"Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M." |
| Journal name |
Proteomics Clin Appl
|
| Publication year |
2010 |
| Sample |
Urine |
| Sample name |
Urine - Rats of liver injury |
| Isolation/purification methods |
Differential centrifugation Filtration UltracentrifugationSucrose cushion Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
Protein-protein interactions for Anpep |
| |
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
|
No interactions are found.
|
|
Pathways in which Anpep is involved |
|
|
|