Gene description for Bhmt
Gene name betaine-homocysteine S-methyltransferase
Gene symbol Bhmt
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Bhmt
ExoCarta ExoCarta_81508
Vesiclepedia VP_81508
Entrez Gene 81508
UniProt O09171  
 Bhmt identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Bhmt
Molecular Function
    methyltransferase activity GO:0008168 IDA
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    identical protein binding GO:0042802 IDA
    protein-containing complex binding GO:0044877 IDA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IBA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IDA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IEA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IMP
    betaine-homocysteine S-methyltransferase activity GO:0047150 ISO
Biological Process
    protein methylation GO:0006479 IDA
    amino-acid betaine metabolic process GO:0006577 IEA
    amino-acid betaine metabolic process GO:0006577 ISO
    amino-acid betaine catabolic process GO:0006579 IEA
    methionine biosynthetic process GO:0009086 IDA
    methionine biosynthetic process GO:0009086 ISO
    methylation GO:0032259 IEA
    S-adenosylmethionine metabolic process GO:0046500 ISO
    'de novo' L-methionine biosynthetic process GO:0071266 IEA
    'de novo' L-methionine biosynthetic process GO:0071266 ISO
    L-methionine salvage GO:0071267 IBA
    L-methionine salvage GO:0071267 IEA
    L-methionine salvage GO:0071267 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 IDA
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Bhmt in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Bhmt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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