Gene description for Slc16a3
Gene name solute carrier family 16 (monocarboxylic acid transporters), member 3
Gene symbol Slc16a3
Other names/aliases Mct3
Mct4
Species Mus musculus
 Database cross references - Slc16a3
ExoCarta ExoCarta_80879
Vesiclepedia VP_80879
Entrez Gene 80879
UniProt P57787  
 Slc16a3 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Slc16a3
Molecular Function
    lactate transmembrane transporter activity GO:0015129 IMP
    lactate:proton symporter activity GO:0015650 IBA
    lactate:proton symporter activity GO:0015650 ISO
    lactate:proton symporter activity GO:0015650 ISS
    pyruvate transmembrane transporter activity GO:0050833 ISO
    pyruvate transmembrane transporter activity GO:0050833 ISS
Biological Process
    lactate transmembrane transport GO:0035873 ISO
    lactate transmembrane transport GO:0035873 ISS
    plasma membrane lactate transport GO:0035879 ISO
    pyruvate catabolic process GO:0042867 IMP
    pyruvate transmembrane transport GO:1901475 ISO
    pyruvate transmembrane transport GO:1901475 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 ISO
    basolateral plasma membrane GO:0016323 ISS
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    lateral plasma membrane GO:0016328 IEA
    lateral plasma membrane GO:0016328 ISO
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
    parallel fiber to Purkinje cell synapse GO:0098688 ISO
    postsynaptic density membrane GO:0098839 ISO
 Experiment description of studies that identified Slc16a3 in sEVs
1
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Slc16a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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