Gene description for Slc16a3
Gene name solute carrier family 16 (monocarboxylic acid transporters), member 3
Gene symbol Slc16a3
Other names/aliases Mct3
Mct4
Species Mus musculus
 Database cross references - Slc16a3
ExoCarta ExoCarta_80879
Vesiclepedia VP_80879
Entrez Gene 80879
UniProt P57787  
 Slc16a3 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Slc16a3
Molecular Function
    lactate transmembrane transporter activity GO:0015129 IMP
    lactate:proton symporter activity GO:0015650 IBA
    lactate:proton symporter activity GO:0015650 ISO
    lactate:proton symporter activity GO:0015650 ISS
    pyruvate transmembrane transporter activity GO:0050833 ISO
    pyruvate transmembrane transporter activity GO:0050833 ISS
Biological Process
    lactate transmembrane transport GO:0035873 ISO
    lactate transmembrane transport GO:0035873 ISS
    plasma membrane lactate transport GO:0035879 ISO
    pyruvate catabolic process GO:0042867 IMP
    pyruvate transmembrane transport GO:1901475 ISO
    pyruvate transmembrane transport GO:1901475 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 ISO
    basolateral plasma membrane GO:0016323 ISS
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    lateral plasma membrane GO:0016328 IEA
    lateral plasma membrane GO:0016328 ISO
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
    parallel fiber to Purkinje cell synapse GO:0098688 ISO
    postsynaptic density membrane GO:0098839 ISO
 Experiment description of studies that identified Slc16a3 in sEVs
1
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Slc16a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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