Gene description for Apobec3
Gene name apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3
Gene symbol Apobec3
Other names/aliases Arp3
BC003314
Cem15
Rfv3
Species Mus musculus
 Database cross references - Apobec3
ExoCarta ExoCarta_80287
Vesiclepedia VP_80287
Entrez Gene 80287
 Apobec3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Apobec3
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISO
    cytidine deaminase activity GO:0004126 IBA
    cytidine deaminase activity GO:0004126 ISO
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    hydrolase activity GO:0016787 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    hematopoietic progenitor cell differentiation GO:0002244 IGI
    transposable element silencing GO:0010526 IDA
    cytidine to uridine editing GO:0016554 IBA
    negative regulation of viral genome replication GO:0045071 IDA
    innate immune response GO:0045087 IEA
    negative regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045869 IBA
    regulation of defense response to virus GO:0050688 IMP
    defense response to virus GO:0051607 IBA
    defense response to virus GO:0051607 IEA
    DNA cytosine deamination GO:0070383 IBA
    regulation of viral life cycle GO:1903900 IMP
Subcellular Localization
    P-body GO:0000932 IBA
    P-body GO:0000932 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Apobec3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Apobec3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dnd1  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Apobec3 is involved
PathwayEvidenceSource
Formation of the Editosome IEA Reactome
Metabolism of RNA IEA Reactome
mRNA Editing IEA Reactome
mRNA Editing: C to U Conversion IEA Reactome





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