Gene description for SPEF2
Gene name sperm flagellar 2
Gene symbol SPEF2
Other names/aliases CT122
KPL2
Species Homo sapiens
 Database cross references - SPEF2
ExoCarta ExoCarta_79925
Vesiclepedia VP_79925
Entrez Gene 79925
HGNC 26293
MIM 610172
UniProt Q9C093  
 SPEF2 identified in sEVs derived from the following tissue/cell type
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for SPEF2
Biological Process
    epithelial cilium movement involved in extracellular fluid movement GO:0003351 ISS
    spermatogenesis GO:0007283 ISS
    sperm axoneme assembly GO:0007288 IBA
    sperm axoneme assembly GO:0007288 IMP
    sperm axoneme assembly GO:0007288 ISS
    skeletal system morphogenesis GO:0048705 ISS
    brain morphogenesis GO:0048854 ISS
    respiratory system development GO:0060541 ISS
Subcellular Localization
    manchette GO:0002177 IBA
    manchette GO:0002177 ISS
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 ISS
    sperm flagellum GO:0036126 IDA
    sperm midpiece GO:0097225 IBA
    sperm midpiece GO:0097225 ISS
 Experiment description of studies that identified SPEF2 in sEVs
1
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPEF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYCBP 26292
Affinity Capture-MS Homo sapiens
2 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
3 TSC22D2 9819
Two-hybrid Homo sapiens
4 APOA1 335
Affinity Capture-MS Homo sapiens
5 CKB 1152
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which SPEF2 is involved
No pathways found





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