Gene description for CALB1
Gene name calbindin 1, 28kDa
Gene symbol CALB1
Other names/aliases CALB
D-28K
Species Homo sapiens
 Database cross references - CALB1
ExoCarta ExoCarta_793
Vesiclepedia VP_793
Entrez Gene 793
HGNC 1434
MIM 114050
UniProt P05937  
 CALB1 identified in sEVs derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for CALB1
Molecular Function
    vitamin D binding GO:0005499 IEA
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    calcium ion binding GO:0005509 IMP
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IMP
    calcium ion binding involved in regulation of presynaptic cytosolic calcium ion concentration GO:0099534 IEA
    calcium ion binding involved in regulation of postsynaptic cytosolic calcium ion concentration GO:0099567 IEA
Biological Process
    short-term memory GO:0007614 IMP
    long-term memory GO:0007616 IMP
    locomotory behavior GO:0007626 IEA
    retina layer formation GO:0010842 IEA
    response to auditory stimulus GO:0010996 IEA
    metanephric part of ureteric bud development GO:0035502 IEA
    metanephric collecting duct development GO:0072205 IEA
    metanephric distal convoluted tubule development GO:0072221 IEA
    metanephric connecting tubule development GO:0072286 IEA
    cochlea development GO:0090102 IEA
    regulation of presynaptic cytosolic calcium ion concentration GO:0099509 IBA
    regulation of postsynaptic cytosolic calcium ion concentration GO:0099566 IEA
    regulation of long-term synaptic potentiation GO:1900271 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    axon GO:0030424 IDA
    axon GO:0030424 ISS
    dendrite GO:0030425 IBA
    dendrite GO:0030425 IDA
    stereocilium GO:0032420 IEA
    cuticular plate GO:0032437 IEA
    neuronal cell body GO:0043025 IDA
    terminal bouton GO:0043195 IBA
    dendritic spine GO:0043197 IBA
    calyx of Held GO:0044305 IEA
    synapse GO:0045202 IBA
    extracellular exosome GO:0070062 HDA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IEA
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
    presynaptic cytosol GO:0099523 IEA
    postsynaptic cytosol GO:0099524 IEA
 Experiment description of studies that identified CALB1 in sEVs
1
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CALB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IQCB1  
Affinity Capture-MS Homo sapiens
2 RANBP9 10048
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 IKBKG 8517
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
4 GRIPAP1 56850
Affinity Capture-MS Homo sapiens
5 IMPA1 3612
Protein-peptide Homo sapiens
6 CACNA1D  
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CALB1 is involved
PathwayEvidenceSource
Amyloid fiber formation TAS Reactome
Metabolism of proteins TAS Reactome





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