Gene description for Wasl
Gene name Wiskott-Aldrich syndrome-like (human)
Gene symbol Wasl
Other names/aliases 2900021I12Rik
3110031I02Rik
N-WASP
Species Mus musculus
 Database cross references - Wasl
ExoCarta ExoCarta_73178
Entrez Gene 73178
UniProt Q91YD9  
 Wasl identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wasl
Molecular Function
    identical protein binding GO:0042802 ISO
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
Biological Process
    membrane invagination GO:0010324 ISO
    vesicle transport along actin filament GO:0030050 ISO
    regulation of protein localization GO:0032880 IMP
    actin nucleation GO:0045010 TAS
    positive regulation of clathrin-mediated endocytosis GO:2000370 ISO
    spindle localization GO:0051653 IMP
    membrane budding GO:0006900 ISO
    transcription, DNA-templated GO:0006351 IEA
    cell cycle GO:0007049 IEA
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IEA
    response to bacterium GO:0009617 IMP
    actin cytoskeleton organization GO:0030036 IMP
    actin filament organization GO:0007015 IEA
    positive regulation of filopodium assembly GO:0051491 ISO
    cell division GO:0051301 IEA
    mitotic nuclear division GO:0007067 IEA
    membrane tubulation GO:0097320 ISO
    cell projection assembly GO:0030031 IMP
    cellular protein complex localization GO:0034629 IMP
    vesicle organization GO:0016050 ISO
    regulation of transcription, DNA-templated GO:0006355 IEA
Subcellular Localization
    cytoplasmic membrane-bounded vesicle GO:0016023 ISO
    lamellipodium GO:0030027 IDA
    actin cap GO:0030478 IDA
    cytoplasm GO:0005737 IC
    Golgi membrane GO:0000139 ISO
    nucleus GO:0005634 IEA
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified Wasl in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wasl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRPF40A  
Reconstituted Complex Mus musculus
View the network image/svg+xml



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