Gene description for Slc39a4
Gene name solute carrier family 39 (zinc transporter), member 4
Gene symbol Slc39a4
Other names/aliases 1600025H15Rik
AU041686
AWMS2
ZIP4
Species Mus musculus
 Database cross references - Slc39a4
ExoCarta ExoCarta_72027
Vesiclepedia VP_72027
Entrez Gene 72027
UniProt Q78IQ7  
 Slc39a4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc39a4
Molecular Function
    zinc ion transmembrane transporter activity GO:0005385 IBA
    zinc ion transmembrane transporter activity GO:0005385 IDA
    zinc ion transmembrane transporter activity GO:0005385 ISO
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 ISO
    zinc ion sensor activity GO:0106219 ISO
    zinc ion sensor activity GO:0106219 ISS
    monoatomic cation:bicarbonate symporter activity GO:0140410 IBA
    zinc ion sequestering activity GO:0140486 IEA
    zinc ion sequestering activity GO:0140486 ISO
Biological Process
    intracellular zinc ion homeostasis GO:0006882 IDA
    intracellular monoatomic cation homeostasis GO:0030003 IBA
    cellular response to zinc ion starvation GO:0034224 IDA
    zinc ion transmembrane transport GO:0071577 IEA
    zinc ion transmembrane transport GO:0071577 ISO
    zinc ion import across plasma membrane GO:0071578 IBA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    apical plasma membrane GO:0016324 IDA
    cytoplasmic vesicle GO:0031410 ISO
    recycling endosome membrane GO:0055038 IDA
    recycling endosome membrane GO:0055038 ISO
 Experiment description of studies that identified Slc39a4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
3
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc39a4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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