Gene description for Ist1
Gene name increased sodium tolerance 1 homolog (yeast)
Gene symbol Ist1
Other names/aliases 2400003C14Rik
AW536298
mKIAA0174
Species Mus musculus
 Database cross references - Ist1
ExoCarta ExoCarta_71955
Vesiclepedia VP_71955
Entrez Gene 71955
UniProt Q9CX00  
 Ist1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ist1
Molecular Function
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 ISO
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    MIT domain binding GO:0090541 IEA
    MIT domain binding GO:0090541 ISO
Biological Process
    protein localization GO:0008104 IBA
    protein localization GO:0008104 ISO
    abscission GO:0009838 ISO
    protein transport GO:0015031 IEA
    viral release from host cell GO:0019076 ISO
    establishment of protein localization GO:0045184 ISO
    positive regulation of proteolysis GO:0045862 IEA
    positive regulation of proteolysis GO:0045862 ISO
    viral capsid secondary envelopment GO:0046745 ISO
    collateral sprouting GO:0048668 IGI
    positive regulation of collateral sprouting GO:0048672 IGI
    cell division GO:0051301 ISO
    cytoskeleton-dependent cytokinesis GO:0061640 ISO
    midbody abscission GO:0061952 IEA
Subcellular Localization
    chromatin GO:0000785 IEA
    chromatin GO:0000785 ISO
    nuclear envelope GO:0005635 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    midbody GO:0030496 ISO
    cytoplasmic vesicle GO:0031410 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    extracellular exosome GO:0070062 ISO
    Flemming body GO:0090543 IEA
    Flemming body GO:0090543 ISO
 Experiment description of studies that identified Ist1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ist1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vps4b 20479
Co-fractionation Mus musculus
2 Spast  
Co-fractionation Mus musculus
3 2610002M06Rik  
Co-fractionation Mus musculus
4 Chmp1a  
Co-fractionation Mus musculus
View the network image/svg+xml



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