Gene description for Isyna1
Gene name myo-inositol 1-phosphate synthase A1
Gene symbol Isyna1
Other names/aliases 1300017C10Rik
AU018670
Species Mus musculus
 Database cross references - Isyna1
ExoCarta ExoCarta_71780
Vesiclepedia VP_71780
Entrez Gene 71780
UniProt Q9JHU9  
 Isyna1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Isyna1
Molecular Function
    inositol-3-phosphate synthase activity GO:0004512 IBA
    inositol-3-phosphate synthase activity GO:0004512 IEA
    inositol-3-phosphate synthase activity GO:0004512 ISO
Biological Process
    inositol biosynthetic process GO:0006021 IBA
    inositol biosynthetic process GO:0006021 IEA
    inositol biosynthetic process GO:0006021 ISO
    phospholipid biosynthetic process GO:0008654 IEA
    negative regulation of inositol biosynthetic process GO:1900089 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Isyna1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Isyna1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Isyna1 is involved
PathwayEvidenceSource
Inositol phosphate metabolism IEA Reactome
Metabolism IEA Reactome
Synthesis of IP2, IP, and Ins in the cytosol IEA Reactome





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