Gene description for Basp1
Gene name brain abundant, membrane attached signal protein 1
Gene symbol Basp1
Other names/aliases 2610024P12Rik
CAP-23
CAP23
Ckap3
NAP-22
NAP22
Species Mus musculus
 Database cross references - Basp1
ExoCarta ExoCarta_70350
Vesiclepedia VP_70350
Entrez Gene 70350
UniProt Q91XV3  
 Basp1 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Microglia 16081791    
 Gene ontology annotations for Basp1
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IBA
    transcription cis-regulatory region binding GO:0000976 IDA
    transcription corepressor activity GO:0003714 IBA
    transcription corepressor activity GO:0003714 ISO
    transcription corepressor activity GO:0003714 ISS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    calmodulin binding GO:0005516 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
Biological Process
    negative regulation of DNA-templated transcription GO:0045892 IBA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISS
    protein homooligomerization GO:0051260 IEA
    protein homooligomerization GO:0051260 ISO
    podocyte differentiation GO:0072112 IDA
    supramolecular fiber organization GO:0097435 IEA
    supramolecular fiber organization GO:0097435 ISO
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    COP9 signalosome GO:0008180 IEA
    COP9 signalosome GO:0008180 ISO
    nuclear matrix GO:0016363 IEA
    nuclear matrix GO:0016363 ISO
    PML body GO:0016605 IEA
    PML body GO:0016605 ISO
    nuclear speck GO:0016607 IDA
    cell junction GO:0030054 IEA
    cell junction GO:0030054 ISO
    growth cone GO:0030426 IEA
 Experiment description of studies that identified Basp1 in sEVs
1
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Basp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
3 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
4 Tfe3  
Affinity Capture-MS Mus musculus
5 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
6 Otx2  
Affinity Capture-MS Mus musculus
7 Epb4.1l2 13822
Co-fractionation Mus musculus
8 Tmod1  
Co-fractionation Mus musculus
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