Gene description for Slc44a4
Gene name solute carrier family 44, member 4
Gene symbol Slc44a4
Other names/aliases 2210409B01Rik
NG22
Species Mus musculus
 Database cross references - Slc44a4
ExoCarta ExoCarta_70129
Vesiclepedia VP_70129
Entrez Gene 70129
UniProt Q91VA1  
 Slc44a4 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Slc44a4
Molecular Function
    choline transmembrane transporter activity GO:0015220 ISO
    choline transmembrane transporter activity GO:0015220 ISS
    antiporter activity GO:0015297 IEA
    thiamine pyrophosphate transmembrane transporter activity GO:0090422 IBA
    thiamine pyrophosphate transmembrane transporter activity GO:0090422 IDA
    thiamine pyrophosphate transmembrane transporter activity GO:0090422 ISO
Biological Process
    acetylcholine biosynthetic process GO:0008292 ISO
    acetylcholine biosynthetic process GO:0008292 ISS
    choline transport GO:0015871 IBA
    choline transport GO:0015871 ISO
    choline transport GO:0015871 ISS
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of cell growth GO:0030307 ISS
    thiamine pyrophosphate transmembrane transport GO:0030974 IBA
    thiamine pyrophosphate transmembrane transport GO:0030974 IDA
    thiamine pyrophosphate transmembrane transport GO:0030974 ISO
    otolith formation GO:0032475 ISS
    neuromast hair cell development GO:0035675 ISS
    acetylcholine secretion GO:0061526 ISO
    acetylcholine secretion GO:0061526 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    apical plasma membrane GO:0016324 ISO
    apical plasma membrane GO:0016324 ISS
 Experiment description of studies that identified Slc44a4 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Slc44a4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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