Gene description for Oxct1
Gene name 3-oxoacid CoA transferase 1
Gene symbol Oxct1
Other names/aliases SCOT
Species Rattus norvegicus
 Database cross references - Oxct1
ExoCarta ExoCarta_690163
Vesiclepedia VP_690163
Entrez Gene 690163
UniProt B2GV06  
 Oxct1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Oxct1
Molecular Function
    succinyl-CoA:3-oxo-acid CoA-transferase activity GO:0008260 IBA
    succinyl-CoA:3-oxo-acid CoA-transferase activity GO:0008260 IDA
    succinyl-CoA:3-oxo-acid CoA-transferase activity GO:0008260 IEA
    succinyl-CoA:3-oxo-acid CoA-transferase activity GO:0008260 ISO
    CoA-transferase activity GO:0008410 IEA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 ISO
Biological Process
    heart development GO:0007507 IEP
    response to nutrient GO:0007584 IEP
    response to xenobiotic stimulus GO:0009410 IEP
    response to hormone GO:0009725 IEP
    response to activity GO:0014823 IEP
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 IMP
    ketone catabolic process GO:0042182 IEA
    ketone catabolic process GO:0042182 ISO
    response to starvation GO:0042594 IEP
    response to ethanol GO:0045471 IEP
    ketone body catabolic process GO:0046952 IEA
    ketone body catabolic process GO:0046952 ISO
    adipose tissue development GO:0060612 IEP
    ketone body metabolic process GO:1902224 IBA
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Oxct1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Oxct1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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