Gene description for Lrrc16a
Gene name leucine rich repeat containing 16A
Gene symbol Lrrc16a
Other names/aliases 1110037D04Rik
AI425970
CARMIL
D130057M20
Lrrc16
Species Mus musculus
 Database cross references - Lrrc16a
ExoCarta ExoCarta_68732
Vesiclepedia VP_68732
Entrez Gene 68732
UniProt Q6EDY6  
 Lrrc16a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lrrc16a
Molecular Function
    protein-containing complex binding GO:0044877 IMP
    protein-containing complex binding GO:0044877 ISO
Biological Process
    establishment or maintenance of cell polarity GO:0007163 ISS
    cell migration GO:0016477 IBA
    cell migration GO:0016477 ISO
    cell migration GO:0016477 ISS
    lamellipodium assembly GO:0030032 ISO
    lamellipodium assembly GO:0030032 ISS
    positive regulation of cell migration GO:0030335 IMP
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of cell migration GO:0030335 ISS
    positive regulation of actin filament polymerization GO:0030838 IMP
    ruffle organization GO:0031529 ISO
    ruffle organization GO:0031529 ISS
    regulation of Arp2/3 complex-mediated actin nucleation GO:0034315 IBA
    macropinocytosis GO:0044351 ISO
    macropinocytosis GO:0044351 ISS
    urate metabolic process GO:0046415 ISO
    urate metabolic process GO:0046415 ISS
    positive regulation of stress fiber assembly GO:0051496 IMP
    positive regulation of stress fiber assembly GO:0051496 ISO
    barbed-end actin filament uncapping GO:0051638 IMP
    actin filament network formation GO:0051639 ISO
    actin filament network formation GO:0051639 ISS
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    positive regulation of lamellipodium organization GO:1902745 IMP
    positive regulation of lamellipodium organization GO:1902745 ISO
    negative regulation of barbed-end actin filament capping GO:2000813 IMP
Subcellular Localization
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    lamellipodium GO:0030027 IBA
    lamellipodium GO:0030027 IDA
    lamellipodium GO:0030027 ISO
    lamellipodium GO:0030027 ISS
    cell leading edge GO:0031252 ISO
    filamentous actin GO:0031941 IDA
    macropinosome GO:0044354 ISO
    macropinosome GO:0044354 ISS
    intermediate filament cytoskeleton GO:0045111 ISS
 Experiment description of studies that identified Lrrc16a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lrrc16a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg5  
Affinity Capture-RNA Mus musculus
2 Lnx1  
Affinity Capture-MS Mus musculus
3 Lnx2  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Lrrc16a is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production IEA Reactome
Hemostasis IEA Reactome





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