Gene description for Steap3
Gene name STEAP family member 3
Gene symbol Steap3
Other names/aliases 1010001D01Rik
Tsap6
pHyde
Species Mus musculus
 Database cross references - Steap3
ExoCarta ExoCarta_68428
Vesiclepedia VP_68428
Entrez Gene 68428
UniProt Q8CI59  
 Steap3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Steap3
Molecular Function
    ferric-chelate reductase activity GO:0000293 IDA
    ferric-chelate reductase activity GO:0000293 IMP
    protein binding GO:0005515 IPI
    cupric reductase (NADH) activity GO:0008823 IBA
    cupric reductase (NADH) activity GO:0008823 IDA
    heme binding GO:0020037 ISO
    heme binding GO:0020037 ISS
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    ferric-chelate reductase (NADPH) activity GO:0052851 IBA
    ferric-chelate reductase (NADPH) activity GO:0052851 IDA
    ferric-chelate reductase (NADPH) activity GO:0052851 IMP
    ferric-chelate reductase (NADPH) activity GO:0052851 ISO
    FAD binding GO:0071949 ISO
    FAD binding GO:0071949 ISS
Biological Process
    iron ion transport GO:0006826 IMP
    apoptotic process GO:0006915 IEA
    protein secretion GO:0009306 ISO
    protein secretion GO:0009306 ISO
    protein secretion GO:0009306 ISS
    copper ion import GO:0015677 IBA
    copper ion import GO:0015677 IDA
    copper ion import GO:0015677 IMP
    iron import into cell GO:0033212 IDA
    positive regulation of apoptotic process GO:0043065 ISO
    establishment of localization in cell GO:0051649 IMP
    iron ion import across cell outer membrane GO:0098706 IDA
    iron ion import across cell outer membrane GO:0098706 IMP
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902167 IMP
    exosomal secretion GO:1990182 IMP
Subcellular Localization
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 IBA
    endosome GO:0005768 IDA
    multivesicular body GO:0005771 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    endosome membrane GO:0010008 IMP
 Experiment description of studies that identified Steap3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Steap3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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