Gene description for Lmbrd1
Gene name LMBR1 domain containing 1
Gene symbol Lmbrd1
Other names/aliases 0910001K20Rik
AV347960
Species Mus musculus
 Database cross references - Lmbrd1
ExoCarta ExoCarta_68421
Vesiclepedia VP_68421
Entrez Gene 68421
UniProt Q8K0B2  
 Lmbrd1 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Lmbrd1
Molecular Function
    insulin receptor binding GO:0005158 IDA
    protein binding GO:0005515 IPI
    cobalamin binding GO:0031419 IEA
    clathrin heavy chain binding GO:0032050 IDA
    AP-2 adaptor complex binding GO:0035612 IMP
    protein transporter activity GO:0140318 ISO
Biological Process
    gastrulation GO:0007369 IMP
    insulin receptor internalization GO:0038016 IMP
    protein localization to lysosome GO:0061462 IBA
    protein localization to lysosome GO:0061462 ISO
    protein localization to lysosome GO:0061462 ISS
    clathrin-dependent endocytosis GO:0072583 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    plasma membrane GO:0005886 IDA
    clathrin-coated vesicle GO:0030136 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    clathrin-coated endocytic vesicle GO:0045334 IDA
 Experiment description of studies that identified Lmbrd1 in sEVs
1
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Lmbrd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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