Gene description for Lmbrd1
Gene name LMBR1 domain containing 1
Gene symbol Lmbrd1
Other names/aliases 0910001K20Rik
AV347960
Species Mus musculus
 Database cross references - Lmbrd1
ExoCarta ExoCarta_68421
Vesiclepedia VP_68421
Entrez Gene 68421
UniProt Q8K0B2  
 Lmbrd1 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Lmbrd1
Molecular Function
    insulin receptor binding GO:0005158 IDA
    protein binding GO:0005515 IPI
    cobalamin binding GO:0031419 IEA
    clathrin heavy chain binding GO:0032050 IDA
    AP-2 adaptor complex binding GO:0035612 IMP
    protein transporter activity GO:0140318 ISO
Biological Process
    gastrulation GO:0007369 IMP
    insulin receptor internalization GO:0038016 IMP
    protein localization to lysosome GO:0061462 IBA
    protein localization to lysosome GO:0061462 ISO
    protein localization to lysosome GO:0061462 ISS
    clathrin-dependent endocytosis GO:0072583 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    plasma membrane GO:0005886 IDA
    clathrin-coated vesicle GO:0030136 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    clathrin-coated endocytic vesicle GO:0045334 IDA
 Experiment description of studies that identified Lmbrd1 in sEVs
1
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Lmbrd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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