Gene description for Rpf2
Gene name ribosome production factor 2 homolog (S. cerevisiae)
Gene symbol Rpf2
Other names/aliases 2810470K21Rik
AU040229
AU043242
Bxdc1
Species Mus musculus
 Database cross references - Rpf2
ExoCarta ExoCarta_67239
Vesiclepedia VP_67239
Entrez Gene 67239
UniProt Q9JJ80  
 Rpf2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rpf2
Molecular Function
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISS
    5S rRNA binding GO:0008097 ISO
    5S rRNA binding GO:0008097 ISS
    rRNA binding GO:0019843 IBA
Biological Process
    ribosomal large subunit assembly GO:0000027 IEA
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IBA
    maturation of LSU-rRNA GO:0000470 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    ribosomal large subunit biogenesis GO:0042273 ISO
    ribosomal large subunit biogenesis GO:0042273 ISS
    regulation of signal transduction by p53 class mediator GO:1901796 ISO
    regulation of signal transduction by p53 class mediator GO:1901796 ISS
    protein localization to nucleolus GO:1902570 ISO
    protein localization to nucleolus GO:1902570 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    chromosome GO:0005694 IEA
    chromosome GO:0005694 ISO
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Rpf2 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rpf2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
2 Pik3c3  
Co-fractionation Mus musculus
3 Atg16l1  
Affinity Capture-MS Mus musculus
4 Cpne7  
Co-fractionation Mus musculus
5 Tgfbrap1  
Co-fractionation Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Rpf2 is involved
No pathways found





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