Gene description for Eif2s2
Gene name eukaryotic translation initiation factor 2, subunit 2 (beta)
Gene symbol Eif2s2
Other names/aliases 2810026E11Rik
38kDa
AA408636
AA571381
AA986487
AW822225
D2Ertd303e
EIF2
EIF2B
Species Mus musculus
 Database cross references - Eif2s2
ExoCarta ExoCarta_67204
Vesiclepedia VP_67204
Entrez Gene 67204
UniProt Q99L45  
 Eif2s2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif2s2
Molecular Function
    mRNA binding GO:0003729 IBA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 ISO
    translation initiation factor activity GO:0003743 ISS
    protein binding GO:0005515 IPI
    translation initiation factor binding GO:0031369 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    in utero embryonic development GO:0001701 IMP
    formation of translation preinitiation complex GO:0001731 IBA
    male germ cell proliferation GO:0002176 IMP
    cytoplasmic translational initiation GO:0002183 ISO
    cytoplasmic translational initiation GO:0002183 ISS
    translational initiation GO:0006413 ISO
    male gonad development GO:0008584 IMP
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    eukaryotic translation initiation factor 2 complex GO:0005850 IBA
    eukaryotic translation initiation factor 2 complex GO:0005850 ISO
    eukaryotic translation initiation factor 2 complex GO:0005850 ISS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
 Experiment description of studies that identified Eif2s2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif2s2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Elf5  
Affinity Capture-MS Mus musculus
3 Foxp3  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Upf1 19704
Co-fractionation Mus musculus
6 Eif1 20918
Co-fractionation Mus musculus
7 Eif5b 226982
Co-fractionation Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
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