Gene description for Gp2
Gene name glycoprotein 2 (zymogen granule membrane)
Gene symbol Gp2
Other names/aliases 2310037I18Rik
AV060639
Species Mus musculus
 Database cross references - Gp2
ExoCarta ExoCarta_67133
Vesiclepedia VP_67133
Entrez Gene 67133
UniProt Q9D733  
 Gp2 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Gp2
Molecular Function
    antigen binding GO:0003823 IDA
    antigen binding GO:0003823 ISO
Biological Process
    antigen transcytosis by M cells in mucosal-associated lymphoid tissue GO:0002412 IDA
    antigen transcytosis by M cells in mucosal-associated lymphoid tissue GO:0002412 IMP
    innate immune response GO:0045087 IEA
    establishment of localization in cell GO:0051649 IDA
    establishment of localization in cell GO:0051649 IMP
    neutrophil migration GO:1990266 IBA
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISS
    endosome GO:0005768 IDA
    endosome GO:0005768 ISO
    external side of plasma membrane GO:0009897 ISO
    external side of plasma membrane GO:0009897 ISS
    cell surface GO:0009986 IBA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    zymogen granule membrane GO:0042589 ISO
    zymogen granule membrane GO:0042589 ISS
    membrane raft GO:0045121 IEA
 Experiment description of studies that identified Gp2 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Gp2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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