Gene description for Ddx18
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
Gene symbol Ddx18
Other names/aliases 2310005B10Rik
Species Mus musculus
 Database cross references - Ddx18
ExoCarta ExoCarta_66942
Vesiclepedia VP_66942
Entrez Gene 66942
UniProt Q8K363  
 Ddx18 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ddx18
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 IEA
    RNA helicase activity GO:0003724 IEA
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IBA
    chromatin remodeling GO:0006338 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    chromosome GO:0005694 ISO
    chromosome GO:0005694 ISS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
 Experiment description of studies that identified Ddx18 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ddx18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 JADE1  
Affinity Capture-MS Homo sapiens
2 Atxn1  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Rbm14  
Two-hybrid Mus musculus
5 Cebpa  
Affinity Capture-MS Mus musculus
6 Tfe3  
Affinity Capture-MS Mus musculus
7 Cul3  
Affinity Capture-MS Mus musculus
8 Atg12  
Positive Genetic Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ddx18 is involved
No pathways found





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