Gene description for Hspbp1
Gene name HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
Gene symbol Hspbp1
Other names/aliases 1500019G21Rik
Species Mus musculus
 Database cross references - Hspbp1
ExoCarta ExoCarta_66245
Vesiclepedia VP_66245
Entrez Gene 66245
UniProt Q99P31  
 Hspbp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hspbp1
Molecular Function
    adenyl-nucleotide exchange factor activity GO:0000774 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    molecular sequestering activity GO:0140313 IEA
    molecular sequestering activity GO:0140313 ISO
Biological Process
    positive regulation of protein ubiquitination GO:0031398 IEA
    positive regulation of protein ubiquitination GO:0031398 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
Subcellular Localization
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    endoplasmic reticulum GO:0005783 IBA
 Experiment description of studies that identified Hspbp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hspbp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Iqcb1  
Affinity Capture-MS Mus musculus
2 Wdr4  
Co-fractionation Mus musculus
3 Ogt  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Hspbp1 is involved
No pathways found





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