Gene description for Tmem9
Gene name transmembrane protein 9
Gene symbol Tmem9
Other names/aliases 1500015G18Rik
AW545782
Species Mus musculus
 Database cross references - Tmem9
ExoCarta ExoCarta_66241
Vesiclepedia VP_66241
Entrez Gene 66241
UniProt Q9CR23  
 Tmem9 identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for Tmem9
Molecular Function
    molecular_function GO:0003674 ND
Biological Process
    lysosomal lumen acidification GO:0007042 ISO
    lysosomal lumen acidification GO:0007042 ISS
    protein transport GO:0015031 IEA
    regulation of protein catabolic process GO:0042176 IMP
    endosomal lumen acidification GO:0048388 ISO
    endosomal lumen acidification GO:0048388 ISS
    proton-transporting V-type ATPase complex assembly GO:0070070 ISO
    proton-transporting V-type ATPase complex assembly GO:0070070 ISS
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISS
Subcellular Localization
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISS
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    late endosome GO:0005770 ISO
    late endosome GO:0005770 ISS
    microtubule cytoskeleton GO:0015630 ISO
    multivesicular body membrane GO:0032585 ISO
    multivesicular body membrane GO:0032585 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
 Experiment description of studies that identified Tmem9 in sEVs
1
Experiment ID 1101
MISEV standards
EM
Biophysical techniques
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
GOLGA2|CANX
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1102
MISEV standards
EM
Biophysical techniques
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
GOLGA2|CANX
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
3
Experiment ID 1103
MISEV standards
EM
Biophysical techniques
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
GOLGA2|CANX
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for Tmem9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Tmem9 is involved
No pathways found





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