|
 Gene description for Serpinb1a |
| Gene name |
serine (or cysteine) peptidase inhibitor, clade B, member 1a |
| Gene symbol |
Serpinb1a |
| Other names/aliases |
1190005M04Rik
AI325983
EI
EIA
ELANH2
LEI
M/NEI
MNEI
PI2 |
| Species |
Mus musculus |
| Database cross references - Serpinb1a |
| ExoCarta |
ExoCarta_66222 |
| Vesiclepedia |
VP_66222 |
| Entrez Gene |
66222 |
| UniProt |
Q9D154
|
| Serpinb1a identified in sEVs derived from the following tissue/cell type |
|
Colon cancer cells
|
37309723
|
|
Faeces
|
33431899
|
|
Faeces
|
33431899
|
|
T-cell lymphoma cells
|
37309723
|
| Gene ontology annotations for Serpinb1a |
|
|
| Experiment description of studies that identified Serpinb1a in sEVs |
| 1 |
| Experiment ID |
907 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Colon cancer cells |
| Sample name |
C26 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 2 |
| Experiment ID |
303 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd63|Cd81|Cd9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33431899
|
| Organism |
Mus musculus |
| Experiment description |
High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance |
| Authors |
"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Faeces |
| Sample name |
Intestinal epithelial cells - C57BL/6 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
Lipid
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC |
|
|
| 3 |
| Experiment ID |
304 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd63|Cd81|Cd9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33431899
|
| Organism |
Mus musculus |
| Experiment description |
High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance |
| Authors |
"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Faeces |
| Sample name |
Intestinal epithelial cells - C57BL/6 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
Lipid
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC |
|
|
| 4 |
| Experiment ID |
908 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
T-cell lymphoma cells |
| Sample name |
EL4 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| Protein-protein interactions for Serpinb1a |
|
|
| Pathways in which Serpinb1a is involved |
|
|
|
