Gene description for SLC8A1
Gene name solute carrier family 8 (sodium/calcium exchanger), member 1
Gene symbol SLC8A1
Other names/aliases NCX1
Species Homo sapiens
 Database cross references - SLC8A1
ExoCarta ExoCarta_6546
Vesiclepedia VP_6546
Entrez Gene 6546
HGNC 11068
MIM 182305
UniProt P32418  
 SLC8A1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC8A1
Molecular Function
    calcium:sodium antiporter activity GO:0005432 IBA
    calcium:sodium antiporter activity GO:0005432 IDA
    calcium:sodium antiporter activity GO:0005432 IMP
    calcium:sodium antiporter activity GO:0005432 TAS
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IDA
    cytoskeletal protein binding GO:0008092 IDA
    ankyrin binding GO:0030506 IPI
    ankyrin binding GO:0030506 ISS
    transmembrane transporter binding GO:0044325 ISS
Biological Process
    regulation of the force of heart contraction GO:0002026 IC
    regulation of the force of heart contraction GO:0002026 ISS
    regulation of heart rate GO:0002027 ISS
    monoatomic ion transport GO:0006811 TAS
    intracellular calcium ion homeostasis GO:0006874 ISS
    intracellular sodium ion homeostasis GO:0006883 IDA
    muscle contraction GO:0006936 TAS
    regulation of gene expression GO:0010468 ISS
    regulation of cell communication by electrical coupling GO:0010649 TAS
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 ISS
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 TAS
    regulation of cardiac muscle contraction by calcium ion signaling GO:0010882 TAS
    vascular associated smooth muscle contraction GO:0014829 ISS
    positive regulation of bone mineralization GO:0030501 IMP
    cellular response to reactive oxygen species GO:0034614 IDA
    sodium ion transmembrane transport GO:0035725 IBA
    sodium ion transmembrane transport GO:0035725 IGI
    sodium ion transmembrane transport GO:0035725 IMP
    response to muscle stretch GO:0035994 IMP
    sodium ion export across plasma membrane GO:0036376 IDA
    relaxation of smooth muscle GO:0044557 ISS
    negative regulation of cytosolic calcium ion concentration GO:0051481 ISS
    cardiac muscle cell development GO:0055013 ISS
    calcium ion homeostasis GO:0055074 ISS
    relaxation of cardiac muscle GO:0055119 IC
    relaxation of cardiac muscle GO:0055119 TAS
    cardiac muscle contraction GO:0060048 TAS
    calcium ion transport into cytosol GO:0060402 ISS
    calcium ion import GO:0070509 IDA
    calcium ion transmembrane transport GO:0070588 IGI
    calcium ion transmembrane transport GO:0070588 ISS
    cellular response to caffeine GO:0071313 ISS
    negative regulation of protein serine/threonine kinase activity GO:0071901 ISS
    membrane depolarization during cardiac muscle cell action potential GO:0086012 TAS
    cell communication by electrical coupling involved in cardiac conduction GO:0086064 ISS
    calcium ion transmembrane import into cytosol GO:0097553 TAS
    calcium ion import across plasma membrane GO:0098703 IBA
    sodium ion import across plasma membrane GO:0098719 IDA
    positive regulation of the force of heart contraction GO:0098735 IMP
    calcium ion export GO:1901660 IDA
    regulation of cardiac conduction GO:1903779 TAS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    postsynaptic density GO:0014069 ISS
    intercalated disc GO:0014704 ISS
    intercalated disc GO:0014704 TAS
    Z disc GO:0030018 ISS
    T-tubule GO:0030315 ISS
    T-tubule GO:0030315 TAS
    axon GO:0030424 IBA
    axon GO:0030424 ISS
    dendrite GO:0030425 ISS
    sarcolemma GO:0042383 IBA
    sarcolemma GO:0042383 ISS
    neuronal cell body GO:0043025 ISS
    axon terminus GO:0043679 ISS
    synapse GO:0045202 IDA
    synapse GO:0045202 ISS
    cell periphery GO:0071944 IDA
    postsynapse GO:0098794 IBA
 Experiment description of studies that identified SLC8A1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC8A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CAV3 859
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here