Gene description for Rsad2
Gene name radical S-adenosyl methionine domain containing 2
Gene symbol Rsad2
Other names/aliases Best5
Vig1
Species Rattus norvegicus
 Database cross references - Rsad2
ExoCarta ExoCarta_65190
Vesiclepedia VP_65190
Entrez Gene 65190
UniProt O70600  
 Rsad2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Rsad2
Molecular Function
    catalytic activity GO:0003824 IEA
    lyase activity GO:0016829 IEA
    metal ion binding GO:0046872 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IBA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 ISO
    4 iron, 4 sulfur cluster binding GO:0051539 ISS
Biological Process
    ossification GO:0001503 IDA
    response to virus GO:0009615 ISO
    response to virus GO:0009615 ISS
    regulation of ossification GO:0030278 IDA
    positive regulation of toll-like receptor 7 signaling pathway GO:0034157 IEA
    positive regulation of toll-like receptor 7 signaling pathway GO:0034157 ISO
    positive regulation of toll-like receptor 7 signaling pathway GO:0034157 ISS
    positive regulation of toll-like receptor 9 signaling pathway GO:0034165 IEA
    positive regulation of toll-like receptor 9 signaling pathway GO:0034165 ISO
    positive regulation of toll-like receptor 9 signaling pathway GO:0034165 ISS
    CD4-positive, alpha-beta T cell activation GO:0035710 ISO
    CD4-positive, alpha-beta T cell activation GO:0035710 ISS
    CD4-positive, alpha-beta T cell differentiation GO:0043367 IEA
    CD4-positive, alpha-beta T cell differentiation GO:0043367 ISO
    CD4-positive, alpha-beta T cell differentiation GO:0043367 ISS
    negative regulation of viral genome replication GO:0045071 IEA
    negative regulation of viral genome replication GO:0045071 ISO
    innate immune response GO:0045087 IEA
    negative regulation of protein secretion GO:0050709 IEA
    negative regulation of protein secretion GO:0050709 ISO
    negative regulation of protein secretion GO:0050709 ISS
    positive regulation of immune response GO:0050778 IBA
    defense response to virus GO:0051607 IBA
    defense response to virus GO:0051607 IEA
    defense response to virus GO:0051607 ISO
    defense response to virus GO:0051607 ISS
    positive regulation of T-helper 2 cell cytokine production GO:2000553 IEA
    positive regulation of T-helper 2 cell cytokine production GO:2000553 ISO
    positive regulation of T-helper 2 cell cytokine production GO:2000553 ISS
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IEA
    mitochondrial outer membrane GO:0005741 ISO
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial inner membrane GO:0005743 ISO
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    Golgi apparatus GO:0005794 IEA
    lipid droplet GO:0005811 IEA
    lipid droplet GO:0005811 ISO
    lipid droplet GO:0005811 ISS
 Experiment description of studies that identified Rsad2 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rsad2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rsad2 is involved
No pathways found





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