Gene description for Ppib
Gene name peptidylprolyl isomerase B
Gene symbol Ppib
Other names/aliases CypB
Scylp
Species Rattus norvegicus
 Database cross references - Ppib
ExoCarta ExoCarta_64367
Vesiclepedia VP_64367
Entrez Gene 64367
UniProt P24368  
 Ppib identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ppib
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IEA
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 ISO
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 ISS
    protein binding GO:0005515 IPI
    cyclosporin A binding GO:0016018 IBA
    RNA polymerase binding GO:0070063 ISO
    RNA polymerase II CTD heptapeptide repeat P3 isomerase activity GO:0140839 IEA
    RNA polymerase II CTD heptapeptide repeat P6 isomerase activity GO:0140840 IEA
Biological Process
    protein peptidyl-prolyl isomerization GO:0000413 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein folding GO:0006457 IBA
    protein folding GO:0006457 IEA
    neutrophil chemotaxis GO:0030593 ISO
    positive regulation of multicellular organism growth GO:0040018 ISO
    positive regulation by host of viral process GO:0044794 ISO
    positive regulation by host of viral genome replication GO:0044829 ISO
    protein stabilization GO:0050821 ISO
    nuclear transport GO:0051169 TAS
    bone development GO:0060348 ISO
    chaperone-mediated protein folding GO:0061077 ISO
    chaperone-mediated protein folding GO:0061077 ISO
    chaperone-mediated protein folding GO:0061077 ISS
    regulation of post-translational protein modification GO:1901873 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum lumen GO:0005788 IEA
    smooth endoplasmic reticulum GO:0005790 IDA
    protein-containing complex GO:0032991 ISS
    endoplasmic reticulum chaperone complex GO:0034663 ISO
    melanosome GO:0042470 IEA
    intracellular membrane-bounded organelle GO:0043231 IBA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Ppib in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ppib
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snca  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Ppib is involved
No pathways found





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