Gene description for Arf1
Gene name ADP-ribosylation factor 1
Gene symbol Arf1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Arf1
ExoCarta ExoCarta_64310
Vesiclepedia VP_64310
Entrez Gene 64310
UniProt P84079  
 Arf1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Arf1
Molecular Function
    magnesium ion binding GO:0000287 IDA
    magnesium ion binding GO:0000287 ISO
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 IDA
    protein domain specific binding GO:0019904 ISO
    phospholipase D activator activity GO:1990583 IDA
Biological Process
    regulation of receptor internalization GO:0002090 IDA
    intracellular copper ion homeostasis GO:0006878 ISO
    intracellular protein transport GO:0006886 IBA
    actin filament organization GO:0007015 IMP
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 IEA
    vesicle-mediated transport GO:0016192 ISO
    regulation of Arp2/3 complex-mediated actin nucleation GO:0034315 IDA
    very-low-density lipoprotein particle assembly GO:0034379 IMP
    positive regulation of endocytosis GO:0045807 IMP
    positive regulation of calcium ion-dependent exocytosis GO:0045956 IDA
    positive regulation of protein secretion GO:0050714 IMP
    Golgi to transport vesicle transport GO:0055108 IDA
    long-term synaptic depression GO:0060292 IDA
    positive regulation of dendritic spine development GO:0060999 IMP
    synaptic vesicle budding GO:0070142 IMP
    dendritic spine organization GO:0097061 IMP
    lysosomal membrane organization GO:0097212 IDA
    postsynaptic actin cytoskeleton organization GO:0098974 IDA
    postsynaptic actin cytoskeleton organization GO:0098974 IMP
    positive regulation of sodium ion transmembrane transport GO:1902307 IMP
    positive regulation of late endosome to lysosome transport GO:1902824 IMP
    positive regulation of ER to Golgi vesicle-mediated transport GO:1902953 IMP
    regulation of phospholipid metabolic process GO:1903725 IDA
    mitotic cleavage furrow ingression GO:1990386 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    cytoplasm GO:0005737 IBA
    late endosome GO:0005770 IDA
    peroxisomal membrane GO:0005778 IDA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    endomembrane system GO:0012505 ISO
    postsynaptic density GO:0014069 IDA
    postsynaptic density GO:0014069 IMP
    sarcomere GO:0030017 ISO
    COPI-coated vesicle GO:0030137 IDA
    cell leading edge GO:0031252 ISO
    protein-containing complex GO:0032991 ISO
    neuron projection GO:0043005 IEA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Arf1 in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Arf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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